The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: A scintigraphic study in rats

The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: A scintigraphic study in rats

Autor Prado, Maria José Brandão de Almeida Autor UNIFESP Google Scholar
Nicastri, Ana Lucia Google Scholar
Costa, Pedro LA Google Scholar
Rockman, T. Google Scholar
Tersariol, Ivame Luis dos Santos Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Barros, Rubens Toledo Google Scholar
Prado, Eduardo BA Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo The aim of the present study was to evaluate renal and liver distribution of two monoclonal immunoglobulin light chains. the chains were purified individually from the urine of patients with multiple myeloma and characterized as lambda light chains with a molecular mass of 28 kDa. They were named BJg (high amount of galactose residues exposed) and BJs (sialic acid residues exposed) on the basis of carbohydrate content. A scintigraphic study was performed on male Wistar rats weighing 250 g for 60 min after iv administration of 1 mg of each protein (7.4 MBq), as the intact proteins and also after carbohydrate oxidation. Images were obtained with a Siemens gamma camera with a high-resolution collimator and processed with a MicroDelta system. Hepatic and renal distribution were established and are reported as percent of injected dose. Liver uptake of BJg was significantly higher than liver uptake of BJs (94.3 vs 81.4%) (P<0.05). This contributed to its greater removal from the intravascular compartment, and consequently lower kidney accumulation of BJg in comparison to BJs (5.7 vs 18.6%) (P<0.05). After carbohydrate oxidation, there was a decrease in hepatic accumulation of both proteins and consequently a higher renal overload. the tissue distribution of periodate-treated BJg was similar to that of native BJs: 82.7 vs 81.4% in the liver and 17.3 vs 18.6% in the kidneys. These observations indicate the important role of sugar residues of Bence Jones proteins for their recognition by specific membrane receptors, which leads to differential tissue accumulation and possible toxicity.
Palavra-chave Bence Jones proteins
scintigraphic study
multiple myeloma
nephrotoxicity
Idioma Inglês
Data de publicação 1997-07-01
Publicado em Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 30, n. 7, p. 865-872, 1997.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Assoc Bras Divulg Cientifica
Extensão 865-872
Fonte http://dx.doi.org/10.1590/S0100-879X1997000700008
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:A1997XN66100008
SciELO S0100-879X1997000700008 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/25751

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