Heparan sulfate-binding peptide promotes the deposition of proteoglycans in the extracellular matrix

Heparan sulfate-binding peptide promotes the deposition of proteoglycans in the extracellular matrix

Autor Colburn, P. Google Scholar
Kobayashi, E. Google Scholar
Buonassisi, V Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo A synthetic peptide, which was shown to bind extracellular matrix heparan sulfate chains with a high degree of affinity and specificity [Colburn el al. (1996): Arch Biochem Biophys 325:129-138], has now been found to promote the transfer and the deposition of endothelial cell surface proteoglycans in the extracellular matrix. the peptide also induces preferential binding of extracellular matrix heparan sulfate proteoglycans, which have been added to the supernatant growth medium, and the requirement for its presence is stringent in that only a negligible amount of proteoglycans are bound to the cell layer in the absence of the peptide. in addition, antibodies directed against the peptide detect the accumulation of the peptide in the matrix compartment where the peptide is found associated with the proteoglycans transferred from the cell surface.The sequence of events induced by the peptide appears to be an extension of a naturally occurring process since proteoglycans with properties similar to those of the species ordinarily present in the extracellular matrix have been observed to transfer from the cell surface to the matrix during a pulse-chase experiment. We suggest that formation of the complex peptide-proteoglycan with consequent displacement of the proteoglycan from its anchorage on the cell. initiates the process of transfer of the heparan sulfate-bound peptide from the cell surface to the extracellular matrix. (C) 1997 Wiley-Liss, Inc.
Palavra-chave endothelial cells
tissue factor pathway inhibitor (TFPI)
heparan sulfate proteoglycans
Idioma Inglês
Data de publicação 1997-06-15
Publicado em Journal of Cellular Biochemistry. New York: Wiley-liss, v. 65, n. 4, p. 574-590, 1997.
ISSN 0730-2312 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 574-590
Fonte http://dx.doi.org/10.1002/(SICI)1097-4644(19970615)65:4<574
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997XC66300012
Endereço permanente http://repositorio.unifesp.br/handle/11600/25741

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