A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi

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dc.contributor.author Suzuki, Erika [UNIFESP]
dc.contributor.author Toledo, Marcos Sergio de [UNIFESP]
dc.contributor.author Takahashi, Helio Kiyoshi [UNIFESP]
dc.contributor.author Straus, Anita Hilda [UNIFESP]
dc.date.accessioned 2016-01-24T12:30:21Z
dc.date.available 2016-01-24T12:30:21Z
dc.date.issued 1997-06-01
dc.identifier http://dx.doi.org/10.1093/glycob/7.4.463
dc.identifier.citation Glycobiology. Oxford: Oxford Univ Press, v. 7, n. 4, p. 463-468, 1997.
dc.identifier.issn 0959-6658
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/25729
dc.description.abstract A mouse monoclonal antibody, MEST-1, was produced against Band 1 glycolipid antigen of Paracoccidioides brasiliensis, the glycan structure of Band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins. the reactivity of MEST-1 MAb was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. Selective oxidation of galactofuranose residues and inhibition assays with different methyl-glycosides, revealed that MAb MEST-1 is directed against the terminal residue of beta-D-gaiactofuranose of Band 1, a phosphoglyceroglycolipid antigen of P.brasiliensis. By indirect immunofluorescence, it was observed that the epitope recognized by MEST-1 is accessible to the antibody in yeast forms of this fungus. Reactivity of MEST-1 with parasites known to express galactofuranose containing glycoconjugates was also analyzed by indirect immunofluorescence. A positive fluorescence was observed with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi, GIPL-1 was identified as the antigen recognized by MEST-1 in Leishmania major, indicating that the MAb MEST-1 recognizes terminal galactofuranose residue in either beta 1-->6 or beta 1-->3 linkage to the mannose. en
dc.format.extent 463-468
dc.language.iso eng
dc.publisher Oxford Univ Press
dc.relation.ispartof Glycobiology
dc.rights Acesso aberto
dc.subject galactofuranose en
dc.subject glycolipid antigen en
dc.subject monoclonal antibody en
dc.subject Paracoccidioides brasiliensis en
dc.subject Leishmania major en
dc.title A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi en
dc.type Artigo
dc.rights.license http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo,DEPT BIOCHEM,EPM,BR-04023900 São Paulo,BRAZIL
dc.description.affiliationUnifesp Universidade Federal de São Paulo,DEPT BIOCHEM,EPM,BR-04023900 São Paulo,BRAZIL
dc.identifier.doi 10.1093/glycob/7.4.463
dc.description.source Web of Science
dc.identifier.wos WOS:A1997XB69300003



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