A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi

A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi

Autor Suzuki, Erika Autor UNIFESP Google Scholar
Toledo, Marcos Sergio de Autor UNIFESP Google Scholar
Takahashi, Helio Kiyoshi Autor UNIFESP Google Scholar
Straus, Anita Hilda Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo A mouse monoclonal antibody, MEST-1, was produced against Band 1 glycolipid antigen of Paracoccidioides brasiliensis, the glycan structure of Band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins. the reactivity of MEST-1 MAb was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. Selective oxidation of galactofuranose residues and inhibition assays with different methyl-glycosides, revealed that MAb MEST-1 is directed against the terminal residue of beta-D-gaiactofuranose of Band 1, a phosphoglyceroglycolipid antigen of P.brasiliensis. By indirect immunofluorescence, it was observed that the epitope recognized by MEST-1 is accessible to the antibody in yeast forms of this fungus. Reactivity of MEST-1 with parasites known to express galactofuranose containing glycoconjugates was also analyzed by indirect immunofluorescence. A positive fluorescence was observed with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi, GIPL-1 was identified as the antigen recognized by MEST-1 in Leishmania major, indicating that the MAb MEST-1 recognizes terminal galactofuranose residue in either beta 1-->6 or beta 1-->3 linkage to the mannose.
Palavra-chave galactofuranose
glycolipid antigen
monoclonal antibody
Paracoccidioides brasiliensis
Leishmania major
Idioma Inglês
Data de publicação 1997-06-01
Publicado em Glycobiology. Oxford: Oxford Univ Press, v. 7, n. 4, p. 463-468, 1997.
ISSN 0959-6658 (Sherpa/Romeo, fator de impacto)
Publicador Oxford Univ Press
Extensão 463-468
Fonte http://dx.doi.org/10.1093/glycob/7.4.463
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:A1997XB69300003
Endereço permanente http://repositorio.unifesp.br/handle/11600/25729

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