A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi

A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi

Author Suzuki, Erika Autor UNIFESP Google Scholar
Toledo, Marcos Sergio de Autor UNIFESP Google Scholar
Takahashi, Helio Kiyoshi Autor UNIFESP Google Scholar
Straus, Anita Hilda Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract A mouse monoclonal antibody, MEST-1, was produced against Band 1 glycolipid antigen of Paracoccidioides brasiliensis, the glycan structure of Band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins. the reactivity of MEST-1 MAb was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. Selective oxidation of galactofuranose residues and inhibition assays with different methyl-glycosides, revealed that MAb MEST-1 is directed against the terminal residue of beta-D-gaiactofuranose of Band 1, a phosphoglyceroglycolipid antigen of P.brasiliensis. By indirect immunofluorescence, it was observed that the epitope recognized by MEST-1 is accessible to the antibody in yeast forms of this fungus. Reactivity of MEST-1 with parasites known to express galactofuranose containing glycoconjugates was also analyzed by indirect immunofluorescence. A positive fluorescence was observed with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi, GIPL-1 was identified as the antigen recognized by MEST-1 in Leishmania major, indicating that the MAb MEST-1 recognizes terminal galactofuranose residue in either beta 1-->6 or beta 1-->3 linkage to the mannose.
Keywords galactofuranose
glycolipid antigen
monoclonal antibody
Paracoccidioides brasiliensis
Leishmania major
Language English
Date 1997-06-01
Published in Glycobiology. Oxford: Oxford Univ Press, v. 7, n. 4, p. 463-468, 1997.
ISSN 0959-6658 (Sherpa/Romeo, impact factor)
Publisher Oxford Univ Press
Extent 463-468
Origin http://dx.doi.org/10.1093/glycob/7.4.463
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1997XB69300003
URI http://repositorio.unifesp.br/handle/11600/25729

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account