Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD

Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD

Author Pacheco, Ana Beatriz Furlanetto Google Scholar
Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Soares, Kellen C C Google Scholar
Almeida, Darcy Fontoura de Google Scholar
Ferreira, Luís Carlos de Souza Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G+C contents. the amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NEVI strains could be assigned to either H1 or H31 serotypes. the results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.
Keywords Escherichia coli
RAPD typing
clonal grouping
serotype
Language English
Date 1997-03-15
Published in Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 148, n. 2, p. 255-260, 1997.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 255-260
Origin http://dx.doi.org/10.1016/S0378-1097(97)00045-1
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1997WP01700022
URI http://repositorio.unifesp.br/handle/11600/25704

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