Purification and primary structure determination of a Bowman-Birk trypsin inhibitor from Torresea cearensis seeds

Purification and primary structure determination of a Bowman-Birk trypsin inhibitor from Torresea cearensis seeds

Autor Tanaka, A. S. Google Scholar
Sampaio, M. U. Google Scholar
Marangoni, S. Google Scholar
deOliveira, B. Google Scholar
Novello, J. C. Google Scholar
Oliva, MLV Google Scholar
Fink, E. Google Scholar
Sampaio, CAM Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
UNIV MUNICH
Resumo A Bowman-Birk-type trypsin inhibitor (TcTl) was purified from seeds of Torresea cearensis, a Brazilian native tree of the Papilionoideae sub-family of Leguminosae, Three forms of the inhibitor were separated by an ion exchange ch ro matography, the major form with 63 amino acids was entirely sequenced; it shows a high structural similarity to the Bowman-Birk inhibitors from other Leguminosae, the putative reactive sites of the inhibitor are a lysine residue at position 15 and a histidine at position 42 as identified by alignment to related inhibitors, direct chemical modification and specific enzymatic degradation, Immunoprecipitation with antibodies raised in rats is reduced significantly if TcTl is complexed with chymotrypsin and, to a lesser degree, if complexed with trypsin, TcTl forms a ternary complex with trypsin and chymotrypsin, the binary complexes with trypsin or chymotrypsin were isolated by gel filtration. Dissociation constants of the complexes with trypsin, plasmin, chymotrypsin, and factor XIIa are 1,36, 50, 1450 nM, respectively; human plasma kallikrein, human factor Xa, porcine pancreatic kallikrein and bovine thrombin are not inhibited, TcTl prolongs blood clotting time of the contact phase activation pathway by inhibition of FXIIa.
Palavra-chave blood clotting
chymotrypsin (EC 3.4.21.1)
factor XII (EC 3.4.21.38)
plasmin (EC 3.4.21.7)
proteinase inhibitor
serine proteinases
trypsin (EC 3.4.21.4)
Idioma Inglês
Data de publicação 1997-03-01
Publicado em Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 378, n. 3-4, p. 273-281, 1997.
ISSN 1431-6730 (Sherpa/Romeo, fator de impacto)
Publicador Walter de Gruyter & Co
Extensão 273-281
Fonte http://dx.doi.org/10.1515/bchm.1997.378.3-4.273
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997WZ88900021
Endereço permanente http://repositorio.unifesp.br/handle/11600/25701

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