Vena cava perfusion in situ: A tool for uptake studies

Vena cava perfusion in situ: A tool for uptake studies

Autor Nagaoka, M. R. Google Scholar
Kouyoumdjian, M. Google Scholar
Borges, D. R. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo While studying the uptake of trypsin and thrombin by the perfused rat liver, we verified that these proteins are internalized neither by hepatocytes nor Kupffer cells. These results raised the possibility that the enzymes might be binding to endothelial cells, either hepatic or vascular. in order to find out if the binding of enzymes to endothelial surface is a liver cell-specific phenomenon, we devised a system to perfuse the rat inferior cava vein in situ. After exsanguination, the vein was perfused with the recirculation of 30 mL of Krebs/BSA solution propellered by a pulsatile flow pump (10 mL/min). the liver was not exsanguinated, but to assure that the organ was indeed excluded from the circuit during the experiment at the end of the perfusion time we added China ink in the perfusion fluid. We verified that trypsin is extracted from the perfusion fluid by the vena cava as efficiently as by the liver, suggesting that the most of the infused trypsin is removed mainly by vascular endothelial cells when the liver perfusion model is used. On the other hand, thrombin is removed mainly by the liver cells since the uptake by the vena cava was insignificant. (C) 1997 Elsevier Science Inc.
Palavra-chave vena cava
liver perfusion
thrombin
trypsin
endocytosis
endothelial cell
Idioma Inglês
Data de publicação 1997-02-01
Publicado em Journal of Pharmacological and Toxicological Methods. New York: Elsevier B.V., v. 37, n. 1, p. 23-26, 1997.
ISSN 1056-8719 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 23-26
Fonte http://dx.doi.org/10.1016/S1056-8719(96)00144-X
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997WP61200004
Endereço permanente http://repositorio.unifesp.br/handle/11600/25690

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