Antigenic similarities to Paracoccidioides brasiliensis in thermo-dependent dimorphic fungi isolated from soil in Botucatu, SP, Brazil

Antigenic similarities to Paracoccidioides brasiliensis in thermo-dependent dimorphic fungi isolated from soil in Botucatu, SP, Brazil

Author Sano, A. Google Scholar
Nishimura, K. Google Scholar
Horie, Y. Google Scholar
Franco, M. Google Scholar
Mendes, R. P. Google Scholar
Coelho, Kunie Iabuki Rabello Google Scholar
Scapolio, SHB Google Scholar
Fukushima, K. Google Scholar
deCamargo, Z. P. Google Scholar
Miyaji, M. Google Scholar
Montenegro, M. R. Google Scholar
Institution NAT HIST MUSEUM & INST
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract We compared the antigenic characteristics of two thermo-dependent dimorphic fungi isolated from soil in Botucatu, an endemic area of paracoccidioidomycosis (PCM) and Paracoccidioides brasiliensis. the soil isolates grew as cerebriform colonies at 37 degrees C (yeast form) and as cottonous colonies at 25 degrees C (mycelial form). No pathogenicity for ddY mice or hamsters were observed. in immunodiffusion test, there were precipitation bands between the 2 soil isolates and pooled PCM patient sera. There were also common precipitation bands at 21, 50 and 58 kDa between the soil isolates antigens and PCM patient sera by Western-blotting, but no gp43 kDa band. No gene for gp43 kDa protein was detected in the soil isolates by PCR. the fact that these isolates were obtained from an endemic area of PCM and there were some antigenic similarities between the soil isolates and P. brasiliensis in immunodiffusion test and Western-blotting may have some importance in epidemiological surveys done with paracoccidioidin as well interfering with the immune response of the exposed population.
Keywords Brazil
dimorphic fungi
ecology
Paracoccidioides brasiliensis
Language English
Date 1997-01-01
Published in Mycopathologia. Dordrecht: Kluwer Academic Publ, v. 138, n. 1, p. 37-41, 1997.
ISSN 0301-486X (Sherpa/Romeo, impact factor)
Publisher Kluwer Academic Publ
Extent 37-41
Origin http://dx.doi.org/10.1023/A:1006835903685
Access rights Closed access
Type Article
Web of Science ID WOS:A1997YK92500007
URI http://repositorio.unifesp.br/handle/11600/25663

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