Sequence of a new Bowman-Birk inhibitor from Torresea acreana seeds and comparison with Torresea cearensis trypsin inhibitor (TcTI2)

Sequence of a new Bowman-Birk inhibitor from Torresea acreana seeds and comparison with Torresea cearensis trypsin inhibitor (TcTI2)

Autor Tanaka, A. S. Google Scholar
Sampaio, M. U. Google Scholar
Mentele, R. Google Scholar
Auerswald, E. A. Google Scholar
Sampaio, CAM Google Scholar
Instituição UNIV MUNICH
Universidade Federal de São Paulo (UNIFESP)
Resumo TaTI (Torresea acreana trypsin inhibitor), a new member of the Bowman-Birk trypsin inhibitor family, was purified from seeds of Torresea acreana, one of the two known species of Torresea, a Brazilian native Leguminosae of the Papilionoideae subfamily. Purification was performed by acetone fractionation, anion-exchange chromatography, and gel filtration. the TaTI appears as M(r) 7000 in SDS-PAGE under reducing conditions. There are 63 amino acid residues present in the TaTI sequence, which was confirmed by mass spectrometry (8388 daltons). the putative reactive sites residues were Lys-15 and Arg-42 at the first and second site, respectively. the antibodies raised against TcTI2, Torresea cearensis trypsin inhibitor 2, showed a cross-reaction with TaTI, but not with other Bowman-Birk inhibitors purified from Leguminosae. the inhibition constants of TaTI and TcTI2 were comparable when measured against trypsin, chymotrypsin, and factor XIIa, but not on plasmin. the latter was tenfold more effectively inhibited by TcTI2 then by TaTI. Neither TaTI nor TcTI2 affects thrombin, plasma kallikrein, or factor Xa.
Palavra-chave Leguminosae
protein sequencing
Torresea acreana
serine proteinase inhibitor
Bowman-Birk inhibitor
Idioma Inglês
Data de publicação 1996-08-01
Publicado em Journal of Protein Chemistry. New York: Plenum Publ Corp, v. 15, n. 6, p. 553-560, 1996.
ISSN 0277-8033 (Sherpa/Romeo, fator de impacto)
Publicador Plenum Publ Corp
Extensão 553-560
Fonte http://dx.doi.org/10.1007/BF01908537
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996VL47000007
Endereço permanente http://repositorio.unifesp.br/handle/11600/25618

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