Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis

Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis

Autor Almeida, Igor C Autor UNIFESP Google Scholar
Neville, DCA Google Scholar
Mehlert, A. Google Scholar
Treumann, A. Google Scholar
Ferguson, MAJ Google Scholar
Previato, J. O. Google Scholar
Travassos, L. R. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
UNIV DUNDEE
Universidade Federal do Rio de Janeiro (UFRJ)
Resumo The major diagnostic antigen of Paracoccinioides brasiliensis is the exocellularly secreted 43,000 Da glycoprotein (gp43) which contains a single N-linked oligosaccharide chain, This oligosaccharide, although poorly immunogenic in man, is responsible for the cross-reactivity of the gp43 with sera from patients with histoplasmosis, and may have a role in fungal virulence, It contains a neutral high-mannose core (Man(7)GlcNAc(2)) to which a (1-->6)-linked alpha-D-Manp chain of variable length, substituted at the 2-O positions by single alpha-D-Manp residues, is attached, A terminal unit of beta-D-galactofuranose is (1-->6)-linked to one of the (1-->2)-linked mannosyl residues, either in the C or in the A arm of the oligosaccharide, the heterogeneity of the oligosaccharide is determined by the different sizes of the A arm and the sites of insertion of the beta-galactofuranosyl unit. the complete structure was determined hy methylation analysis, H-1-NMR, mass spectrometry, acetolysis and mannosidase degradation, Electrospray mass spectrometry showed that the oligosaccharide comprises several subtypes ranging from Hex(18)GlcNAc(2) to Hex(10)GlcNAc(2) which accounts for the diffuse migration of the gp43 in polyacrylamide gels, the average size of the most frequent subtype is Hex(13.6)GlcNAc(2). Dilute acid treatment to remove beta-D-Galf reduced the molecular masses of the majority of the subtypes by a single sugar unit.
Palavra-chave Paracoccidioides brasiliensis
gp43
diagnostic antigen
N-linked oligosaccharide
Idioma Inglês
Data de publicação 1996-07-01
Publicado em Glycobiology. Oxford: Oxford Univ Press United Kingdom, v. 6, n. 5, p. 507-515, 1996.
ISSN 0959-6658 (Sherpa/Romeo, fator de impacto)
Publicador Oxford Univ Press United Kingdom
Extensão 507-515
Fonte http://dx.doi.org/10.1093/glycob/6.5.507
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:A1996VF56600008
Endereço permanente http://repositorio.unifesp.br/handle/11600/25608

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