Use of the in vitro induced antibody production test (IVIAP) to elucidate inconclusive status of HIV-1 infection

Use of the in vitro induced antibody production test (IVIAP) to elucidate inconclusive status of HIV-1 infection

Autor Bellei, N. Google Scholar
Granato, C. Google Scholar
Tomiyama, H. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Studies have demonstrated the diagnostic usefulness of in vitro production of virus-specific antibodies by peripheral blood mononuclear cells (PMBC) from HN-infected subjects. We evaluated the IVIAP (in vitro induced antibody production) by lympocytes from peripheral blood of 30 adult patients. Samples included 10 patients with inconclusive status of HIV-1 infection on previous serologic screening (2 blood donors, 8 with high risk behavior), 10 AIDS patients (CDC II-IV), and 10 known seronegative subjects. Positive IVIAP results were obtained on 2 of 10 inconclusive cases, earlier than seroconversion would demonstrate. the 8 remaining cases resulted in a negative outcome on IVIAP test, evert in 2 seronegative homosexuals presenting Kaposi's sarcoma. Results showed 100% concordance either with a clinical/serological follow-up done after IVIAP tests or PCR performed for Kaposi's cases. Also, no false-positive or false negative was detected on control groups. We concluded that IVIAP could identify the real status of HIV infection in a shorter time and with low cost compared to conventional serological methods currently available. the IVIAP test is easily performed so that can be considered as a routine supplemental test.
Idioma Inglês
Data de publicação 1996-06-01
Publicado em Diagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 25, n. 2, p. 65-69, 1996.
ISSN 0732-8893 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 65-69
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996VG63700002
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