Structural characterization of a new galactofuranose-containing glycolipid antigen of Paracoccidioides brasiliensis

Structural characterization of a new galactofuranose-containing glycolipid antigen of Paracoccidioides brasiliensis

Autor Levery, S. B. Google Scholar
Toledo, M. S. Google Scholar
Suzuki, E. Google Scholar
Salyan, MEK Google Scholar
Hakomori, S. I. Google Scholar
Straus, A. H. Google Scholar
Takahashi, H. K. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
PERKIN ELMER APPL BIOSYST DIV
BIOMEMBRANE INST
Resumo An acidic glycolipid (Band 1), purified from P. brasiliensis by a combination of ion exchange chromatography, HPLC, and HPTLC, was found to be reactive with sera of all patients with paracoccidioidomycosis (PCM). Monosaccharide analysis of Band 1 yielded mannose and galactose in a 2:1 ratio, while mild acid hydrolysis and mild periodate oxidation/(NaBH4)-H-3, reduction indicated the presence of a terminal galactofuranose. Preliminary analysis of H-1-NMR and MS data suggests that the structure of the glycan is Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins (Ins = myo-inositol). Removal of the galacto-furanose decreased by 60-80% the reactivity of sera from PCM patients with Band 1, suggesting that this residue is immunodominant. With the presumed absence of galactofuranose in mammalian hosts, compounds containing this residue may be useful targets for therapy of several parasitic and fungal diseases. (C) 1996 Academic Press, Inc.
Idioma Inglês
Data de publicação 1996-05-15
Publicado em Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Jnl-comp Subscriptions, v. 222, n. 2, p. 639-645, 1996.
ISSN 0006-291X (Sherpa/Romeo, fator de impacto)
Publicador Academic Press Inc Jnl-comp Subscriptions
Extensão 639-645
Fonte http://dx.doi.org/10.1006/bbrc.1996.0796
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996UM27700076
Endereço permanente http://repositorio.unifesp.br/handle/11600/25595

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta