Effect of aqueous and nonaqueous fixatives on the quantitative estimation of collagen-proteoglycan interaction in tissue sections

Effect of aqueous and nonaqueous fixatives on the quantitative estimation of collagen-proteoglycan interaction in tissue sections

Autor Toledo, OMS Google Scholar
Taniwaki, N. N. Google Scholar
Saldiva, PHN Google Scholar
Montes, G. S. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo The present study was designed to assess the influence of aqueous and nonaqueous fixatives on the quantitative estimation of collagen-proteoglycan interaction in tissue sections. Tissues containing different collagen types and distinct sulfated proteoglycan classes were isolated from pig costal cartilage, human skin, and the inner muscular layer of dog small intestine and fixed using aqueous or nonaqueous methods. the results showed that the best fixation method was exposure to paraformaldehyde gas. When using aqueous fixatives, proteoglycans were lost to different degrees among the various tissues analyzed, reflecting differences in chemical properties of proteoglycan classes and/or in their interactions with other matrix components such as collagen.
Palavra-chave collagen
proteoglycans
aqueous fixatives
nonaqueous fixatives
paraformaldehyde
Idioma Inglês
Data de publicação 1996-05-01
Publicado em Biotechnic & Histochemistry. Baltimore: Williams & Wilkins, v. 71, n. 3, p. 109-114, 1996.
ISSN 1052-0295 (Sherpa/Romeo, fator de impacto)
Publicador Williams & Wilkins
Extensão 109-114
Fonte http://dx.doi.org/10.3109/10520299609117145
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996UL57500001
Endereço permanente http://repositorio.unifesp.br/handle/11600/25593

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