Organization of trans-sialidase genes in Trypanosoma cruzi

Organization of trans-sialidase genes in Trypanosoma cruzi

Autor Egima, C. M. Google Scholar
Briones, MRS Google Scholar
Freitas, LHG Google Scholar
Schenkman, RPF Google Scholar
Uemura, H. Google Scholar
Schenkman, S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
NAGASAKI UNIV
Resumo Trypanosoma cruzi trans-sialidase is encoded by a family of genes containing a conserved region, which corresponds to the catalytic and amino-terminal domain of the enzyme. Most, but not all genes, also encode a variable region formed by 12 amino acid repeats at the carboxy-terminus of the protein that are not required for enzymatic activity. To design gene knock-out strategies and understand how trans-sialidase expression is regulated, we have studied the genome organization of trans-sialidase genes. We show here that the different types of trans-sialidase genes are distributed in more than one chromosomal band with sizes ranging from 0.8 to 1.5 Mb pairs in several T. cruzi strains. in the Y-strain, all repeat-containing genes are localized in one chromosomal band of 1.1 Mb, while the repeat-minus genes are in two chromosomes of 0.82 and 0.79 Mb. the repeat-containing genes have similar catalytic and intergenic regions, but variable lengths of the repeated region. the trans-sialidase genes with the repeats are in tandem of up to 12 genes in at least four different clusters. Each cluster contains genes with different numbers of repeats, according to the physical maps of eight independent cosmids, and in the same cluster there are genes that code for active and inactive trans-sialidases. There are 80 +/- 30 copies of the repeat-containing genes grouped in two NotI fragments of 120 and 180 Kb. Therefore, in the Y-strain, the trans-sialidase genes containing repeats might be arranged in three to four clusters in two homologous chromosomes, each cluster having up to 12 genes with different repeat numbers.
Palavra-chave Trypanosoma cruzi
gene
sialidase
chromosome
Idioma Inglês
Data de publicação 1996-05-01
Publicado em Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 77, n. 2, p. 115-125, 1996.
ISSN 0166-6851 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 115-125
Fonte http://dx.doi.org/10.1016/0166-6851(96)02582-0
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996UV96800001
Endereço permanente http://repositorio.unifesp.br/handle/11600/25589

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