Plant serine proteinase inhibitors. Structure and biochemical applications on plasma kallikrein and related enzymes

Plant serine proteinase inhibitors. Structure and biochemical applications on plasma kallikrein and related enzymes

Autor Sampaio, CAM Google Scholar
Oliva, MLV Google Scholar
Sampaio, M. U. Google Scholar
Batista, IFC Google Scholar
Bueno, N. R. Google Scholar
Tanaka, A. S. Google Scholar
Auerswald, E. A. Google Scholar
Fritz, H. Google Scholar
Instituição UNIV MUNICH
Universidade Federal de São Paulo (UNIFESP)
Resumo The action of two Bowman-Birk and several plant Kunitz-type inhibitors were studied on trypsin, chymotrypsin, plasma kallikrein and factor XII. the primary structure of some of them was completely defined. the results showed that the Bowman-Birk type inhibitors, although potent inhibitors for trypsin (K-i in the range of 1-2 nM), are not able to inhibit plasma kallikrein, Factor XII (K-i = 1.4 mu M) and chymotrypsin (K-i = 5.0 nM) are inhibited by Torresea cearensis trypsin inhibitor (TcTI) but not by Dioclea glabra trypsin inhibitor (DgTI). Both inhibitors reactive site regions are highly homologous, and the amino acid residues in P1 position are the same, Lys and His; major differences are in the charge of the C-terminal portion of the molecules, the studied Kunitz-type inhibitors were all able to inhibit plasma kallikrein (Ki between 4 and 80 nM), with the exception of Schizolobium parahyba chymotrypsin inhibitor (SpCI), that is specific for chymotrypsin. All Kunitz-type inhibitors inactivate chymotrypsin, but with a dissociation constant in the range of 0.1 to 0.6 mu M Factor XIIf is inhibited with K-i in the range of 0.1 mu M. Bauhinia bauhinioides trypsin inhibitor (BbTI) did not promote factor XIIf inhibition. the Kunitz-type inhibitors are a highly homologous, sharing 60% identity in the N-terminal portion of the loop containing the reactive site, and 28.6% identity in the C-terminal portion of the same loop.
Idioma Inglês
Data de publicação 1996-05-01
Publicado em Immunopharmacology. Amsterdam: Elsevier B.V., v. 32, n. 1-3, p. 62-66, 1996.
ISSN 0162-3109 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 62-66
Fonte http://dx.doi.org/10.1016/0162-3109(96)00073-2
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996UR64300014
Endereço permanente http://repositorio.unifesp.br/handle/11600/25587

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