Molecular cloning and functional characterization of a mouse bradykinin B1 receptor gene

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dc.contributor.author Pesquero, J. B.
dc.contributor.author Pesquero, J. L.
dc.contributor.author Oliveira, S. M.
dc.contributor.author Roscher, A. A.
dc.contributor.author Metzger, R.
dc.contributor.author Ganten, D.
dc.contributor.author Bader, M.
dc.date.accessioned 2016-01-24T11:40:30Z
dc.date.available 2016-01-24T11:40:30Z
dc.date.issued 1996-03-07
dc.identifier http://dx.doi.org/10.1006/bbrc.1996.0384
dc.identifier.citation Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Jnl-comp Subscriptions, v. 220, n. 1, p. 219-225, 1996.
dc.identifier.issn 0006-291X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/25569
dc.description.abstract The gene encoding a putative mouse bradykinin B1 receptor was cloned from a genomic library by low stringency screening. Analysis of two isolated clones revealed a region which contains an open reading frame uninterrupted by introns and encodes a 334 amino acid protein, which exhibits seven potential transmembrane domains and is 68% identical to the human and rabbit bradykinin B1 receptors, Lipopolysaccharide-treatment induces B1 receptor transcripts in the heart, liver and lung, Stable expression of the coding region in COS-7 cells resulted in high levels of binding sites for the specific B1 ligand des-Arg(10) kallidin (K-d = 1.3 nM; B-max = 51 fmol/mg protein). the rank order of affinity of the receptor for the agonists and antagonists was: des Arg(9)BKdes-Arg(9)Leu(8)BKdrs-Arg(10)kallidin much greater than Hoe-140 = bradykinin. Functional coupling of the cloned receptor was demonstrated by the dose-dependent effect of des-Arg(9)BK on the extracellular acidification rate in stably transfected COS-7 cells. This effect was not produced by bradykinin and could be blocked by the B1 antagonist des-Arg(9)Leu(8)BK. (C) 1996 Academic Press, Inc. en
dc.format.extent 219-225
dc.language.iso eng
dc.publisher Academic Press Inc Jnl-comp Subscriptions
dc.relation.ispartof Biochemical and Biophysical Research Communications
dc.rights Acesso restrito
dc.title Molecular cloning and functional characterization of a mouse bradykinin B1 receptor gene en
dc.type Artigo
dc.contributor.institution MAX DELBRUCK CTR MOLEC MED
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal de Minas Gerais (UFMG)
dc.contributor.institution UNIV MUNICH
dc.contributor.institution CELL CONTROL BIOMED LABS
dc.description.affiliation MAX DELBRUCK CTR MOLEC MED,D-13125 BERLIN,GERMANY
dc.description.affiliation ESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04032062 São Paulo,BRAZIL
dc.description.affiliation UNIV FED MINAS GERAIS,ICB,DEPT PHYSIOL & BIOPHYS,BR-31270010 BELO HORIZONT,MG,BRAZIL
dc.description.affiliation UNIV MUNICH,CHILDRENS HOSP,DEPT CLIN CHEM & BIOCHEM,D-80337 MUNICH,GERMANY
dc.description.affiliation CELL CONTROL BIOMED LABS,D-82152 MARTINSRIED,GERMANY
dc.description.affiliationUnifesp ESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04032062 São Paulo,BRAZIL
dc.identifier.doi 10.1006/bbrc.1996.0384
dc.description.source Web of Science
dc.identifier.wos WOS:A1996UA75700039



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