Molecular cloning and functional characterization of a mouse bradykinin B1 receptor gene

Molecular cloning and functional characterization of a mouse bradykinin B1 receptor gene

Author Pesquero, J. B. Google Scholar
Pesquero, J. L. Google Scholar
Oliveira, S. M. Google Scholar
Roscher, A. A. Google Scholar
Metzger, R. Google Scholar
Ganten, D. Google Scholar
Bader, M. Google Scholar
Institution MAX DELBRUCK CTR MOLEC MED
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Minas Gerais (UFMG)
UNIV MUNICH
CELL CONTROL BIOMED LABS
Abstract The gene encoding a putative mouse bradykinin B1 receptor was cloned from a genomic library by low stringency screening. Analysis of two isolated clones revealed a region which contains an open reading frame uninterrupted by introns and encodes a 334 amino acid protein, which exhibits seven potential transmembrane domains and is 68% identical to the human and rabbit bradykinin B1 receptors, Lipopolysaccharide-treatment induces B1 receptor transcripts in the heart, liver and lung, Stable expression of the coding region in COS-7 cells resulted in high levels of binding sites for the specific B1 ligand des-Arg(10) kallidin (K-d = 1.3 nM; B-max = 51 fmol/mg protein). the rank order of affinity of the receptor for the agonists and antagonists was: des Arg(9)BKdes-Arg(9)Leu(8)BKdrs-Arg(10)kallidin much greater than Hoe-140 = bradykinin. Functional coupling of the cloned receptor was demonstrated by the dose-dependent effect of des-Arg(9)BK on the extracellular acidification rate in stably transfected COS-7 cells. This effect was not produced by bradykinin and could be blocked by the B1 antagonist des-Arg(9)Leu(8)BK. (C) 1996 Academic Press, Inc.
Language English
Date 1996-03-07
Published in Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Jnl-comp Subscriptions, v. 220, n. 1, p. 219-225, 1996.
ISSN 0006-291X (Sherpa/Romeo, impact factor)
Publisher Academic Press Inc Jnl-comp Subscriptions
Extent 219-225
Origin http://dx.doi.org/10.1006/bbrc.1996.0384
Access rights Closed access
Type Article
Web of Science ID WOS:A1996UA75700039
URI http://repositorio.unifesp.br/handle/11600/25569

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