Molecular cloning and functional characterization of a mouse bradykinin B1 receptor gene

Molecular cloning and functional characterization of a mouse bradykinin B1 receptor gene

Autor Pesquero, J. B. Google Scholar
Pesquero, J. L. Google Scholar
Oliveira, S. M. Google Scholar
Roscher, A. A. Google Scholar
Metzger, R. Google Scholar
Ganten, D. Google Scholar
Bader, M. Google Scholar
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Minas Gerais (UFMG)
Resumo The gene encoding a putative mouse bradykinin B1 receptor was cloned from a genomic library by low stringency screening. Analysis of two isolated clones revealed a region which contains an open reading frame uninterrupted by introns and encodes a 334 amino acid protein, which exhibits seven potential transmembrane domains and is 68% identical to the human and rabbit bradykinin B1 receptors, Lipopolysaccharide-treatment induces B1 receptor transcripts in the heart, liver and lung, Stable expression of the coding region in COS-7 cells resulted in high levels of binding sites for the specific B1 ligand des-Arg(10) kallidin (K-d = 1.3 nM; B-max = 51 fmol/mg protein). the rank order of affinity of the receptor for the agonists and antagonists was: des Arg(9)BKdes-Arg(9)Leu(8)BKdrs-Arg(10)kallidin much greater than Hoe-140 = bradykinin. Functional coupling of the cloned receptor was demonstrated by the dose-dependent effect of des-Arg(9)BK on the extracellular acidification rate in stably transfected COS-7 cells. This effect was not produced by bradykinin and could be blocked by the B1 antagonist des-Arg(9)Leu(8)BK. (C) 1996 Academic Press, Inc.
Idioma Inglês
Data 1996-03-07
Publicado em Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Jnl-comp Subscriptions, v. 220, n. 1, p. 219-225, 1996.
ISSN 0006-291X (Sherpa/Romeo, fator de impacto)
Editor Academic Press Inc Jnl-comp Subscriptions
Extensão 219-225
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996UA75700039

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