Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA

Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA

Autor Pacheco, ABF Google Scholar
Guth, BEC Google Scholar
deAlmeida, D. F. Google Scholar
Ferreira, LCS Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Resumo Two enterotoxigenic Escherichia coli (ETEC) strains (H10407 and 4011-1) were characterized by random amplification of polymorphic DNA (RAPD) profiles using 10-mer oligonucleotides with diverse GC content, All tested primers yielded arrays of amplified DNA products ranging in size from 200 to 3000 bp. the effects of annealing temperature, template concentration and GC content of the primers were evaluated and an optimal reaction procedure was established. Application of the RAPD analysis to ten ETEC strains belonging to five different serotypes showed that strains of the same serotype shared identical or almost identical band profiles, suggesting a similar genetic composition. the use of RAPD profiles as a tool in epidemiological analysis of ETEC is discussed.
Palavra-chave ETEC
enteritis
Escherichia coli
RAPD
epidemiological tool
genetic polymorphism
Idioma Inglês
Data de publicação 1996-03-01
Publicado em Research in Microbiology. Paris Cedex 15: Editions Scientifiques Elsevier, v. 147, n. 3, p. 175-182, 1996.
ISSN 0923-2508 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 175-182
Fonte http://dx.doi.org/10.1016/0923-2508(96)80217-8
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996UC60500006
Endereço permanente http://repositorio.unifesp.br/handle/11600/25564

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