Antibodies to bovine serum albumin in Brazilian children and young adults with IDDM

Antibodies to bovine serum albumin in Brazilian children and young adults with IDDM

Author Pardini, V. C. Google Scholar
Vieira, JGH Google Scholar
Miranda, W. Google Scholar
Ferreira, SRC Google Scholar
Velho, G. Google Scholar
Russo, EMK Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE - To evaluate the prevalence of IgG antibodies to bovine serum albumin (BSA) in a cohort of Brazilian children and young adults with IDDM.RESEARCH DESIGN END METHODS - Sera from 81 subjects with <1 year of IDDM (group 1), 111 subjects with >1 year of IDDM (group 2), and 207 normoglycemic subjects were tested using an immunofluorimetric assay. A receiver-operating-characteristic curve was used to establish the threshold of anti-BSA antibody titers defining the positivity of the assay.RESULTS - the distribution of the fluorimetric index (FI) of anti-BSA antibodies did not have a gaussian profile. Rank sum of FI was significantly higher inpatients than in control subjects (P < 0.0001). Average logFI values of both IDDM groups were significantly higher than that of the control group (P < 0.005 for both groups). There was a trend toward higher FI levels in group 1 than in group 2 (P = 0.06). A FI cutoff of 0.7 optimized the ratio of true-positive to false-positive of the assay, with the best equilibrium between sensitivity and specificity. the prevalence of anti-BSA antibodies was 52% in group 1, 47% in group 2, and 28% in the control group (P = 0.0001). An independent association between anti-BSA antibodies and IDDM, with an odds ratio of 3.03 (P < 0.0001), was observed in a logistic regression analysis. However anti-BSA antibodies explained only 5% of the variability of IDDM versus NIDDM.CONCLUSIONS - Our results confirm that the prevalence of anti-BSA antibodies is higher in IDDM subjects than in control subjects, even after 1 year of diabetes. However, a large overlap of antibody titers is observed in patients and control subjects, suggesting that anti-BSA antibodies are neither sensitive nor specific markers of IDDM.
Language English
Date 1996-02-01
Published in Diabetes Care. Alexandria: Amer Diabetes Assoc, v. 19, n. 2, p. 126-129, 1996.
ISSN 0149-5992 (Sherpa/Romeo, impact factor)
Publisher Amer Diabetes Assoc
Extent 126-129
Access rights Closed access
Type Article
Web of Science ID WOS:A1996TR89200004

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