Evaluation of recent techniques for detection of red blood cell antibodies in sera of reference samples, patients, pregnant women, and blood donors

Evaluation of recent techniques for detection of red blood cell antibodies in sera of reference samples, patients, pregnant women, and blood donors

Autor deCastilho, L. M. Google Scholar
Pellegrino, J. Google Scholar
Bechelli, APP Google Scholar
LePennec, P. Y. Google Scholar
Mendes, N. F. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The sensitivity of the low ionic strength solution antiglobulin test (LISS-AGT), polyethylene glycol antiglobulin test (PEG-AGT), low ionic strength solution solid-phase antiglobulin test (LISS-SPAT), gel low ionic strength solution antiglobulin test (GEL-LISS), and gel papain Bst (GEL-PAP) was compared in titration studies of 460 sera containing identified IgG alloantibodies. the GEL-PAP was 100% sensitive to detect Rh antibodies, whereas the PEG-AGT was the most sensitive to detect Kell, Duffy, Kidd, Ss, and rare blood group antibodies. the better performance of PEG-AGT was especially obvious with Kell, Duffy, and Ss antibodies (S = 100%). When the sensitivity of the LISS-AGT, PEG-AGT, GEL-LISS, and GEL-PAP was evaluated in different routines, the GEL-LISS showed to be more sensitive than PEG-AGT in the detection of clinically significant antibodies. These discrepant results showed that the performance of a technique may change when it is applied as a routine. (C) 1996 Wiley-Liss, Inc.
Palavra-chave erythrocyte antibodies
blood transfusion
blood groups
alloantibodies
Idioma Inglês
Data de publicação 1996-01-01
Publicado em Journal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 10, n. 5, p. 250-256, 1996.
ISSN 0887-8013 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 250-256
Fonte http://dx.doi.org/10.1002/(SICI)1098-2825(1996)10:5<250
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1996VH34700004
Endereço permanente http://repositorio.unifesp.br/handle/11600/25546

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