Keloid heterograft in the hamster (Mesocricetus auratus) cheek pouch

Show simple item record

dc.contributor.author Hochman, Bernardo [UNIFESP]
dc.contributor.author Vilas Bôas, Flaviane Cássia [UNIFESP]
dc.contributor.author Mariano, Mario [UNIFESP]
dc.contributor.author Ferreira, Lydia Masako [UNIFESP]
dc.date.accessioned 2015-06-14T13:31:37Z
dc.date.available 2015-06-14T13:31:37Z
dc.date.issued 2005-06-01
dc.identifier http://dx.doi.org/10.1590/S0102-86502005000300003
dc.identifier.citation Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 20, n. 3, p. 200-212, 2005.
dc.identifier.issn 0102-8650
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/2550
dc.description.abstract PURPOSE: To study the integration of keloid heterograft in hamster (Mesocricetus auratus) cheek pouch. METHODS: The sample is formed by 18 male hamsters, heterogenic ones, aged between 10 and 14 weeks. Keloid fragments were obtained from keloid scars of the breast region of adult female mulatto patient. Each hamster received keloid fragments into both of its pouches, in a total of 36 grafted fragments. Animals were distributed into 6 groups for having their grafts assessed in the days 5, 12, 21, 42, 84, and 168. A macroscopic assessment is performed by comparing the pouch containing the grafted fragment, at each time point, with the same pouch in the immediate post surgical moment through a comparison of standardized photographs. Under microscope, the presence of blood vases is considered within the conjunctive tissue of the grafted fragment, as a criterion of its integration. Other events, as keratin secretion, the presence of cellular infiltrated, epithelium and keloid collagen fibers aspects are also analyzed. RESULTS: Macroscopy reveals intensive vascularization of the pouch up to 12 days from the transplantation and the presence of constant dark brown pigmentation on the grafted keloid fragments. In microscopy, the integration of keloid fragments is considered by the presence of blood capillary vases within conjunctive tissue. The presence of intensive cellular inflammatory type infiltrated up to 12 days is also observed, as well as the remaining of keloid epithelium up to 21 days, and the appearing of melanocytes from the day 42. CONCLUSION: Hamster cheek pouch represents, a priori, an experimental model for the investigation of keloid. en
dc.description.abstract OBJETIVO: Investigar a integração do transplante heterólogo de quelóide na bolsa jugal do hamster (Mesocricetus auratus). MÉTODOS: A amostragem consiste de 18 hamsters machos, heterogênicos, com 10 a 14 semanas de idade. Fragmentos de quelóide foram obtidos de cicatrizes queloidianas da região mamária de paciente adulta parda. Cada hamster foi enxertado em ambas as bolsas com fragmentos de quelóide, totalizando 36 fragmentos enxertados. Os animais foram distribuídos em 6 grupos para exame dos fragmentos enxertados, com 5, 12, 21, 42, 84 e 168 dias. Uma avaliação macroscópica é realizada comparando a bolsa contendo o fragmento enxertado em cada período com a mesma bolsa no pós-operatório imediato, mediante a comparação de fotografias padronizadas. À microscopia, considera-se a presença de vasos sangüíneos no tecido conjuntivo do fragmento enxertado como critério de integração do mesmo. Outros eventos, como secreção de queratina, presença de infiltrados celulares e aspecto do epitélio e das fibras colágenas do quelóide, também são observados. RESULTADOS: A macroscopia revela intensa vascularização na bolsa até 12 dias de enxertia, e a presença constante de pigmentação castanho-escura nos fragmentos de quelóide enxertados. Na microscopia constata-se a integração dos fragmentos de quelóide pela presença de capilares sangüíneos no tecido conjuntivo. Observa-se, também, a presença de intenso infiltrado celular do tipo inflamatório até 12 dias, a permanência do epitélio do quelóide até 21 dias, e o aparecimento de melanócitos a partir de 42 dias. CONCLUSÃO: A bolsa jugal do hamster representa, a priori, modelo experimental para investigação do quelóide. pt
dc.format.extent 200-212
dc.language.iso eng
dc.publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Mesocricetus en
dc.subject Transplantation en
dc.subject Skin transplantation en
dc.subject Keloid en
dc.subject Mesocricetus pt
dc.subject Transplante heterólogo pt
dc.subject Transplante de pele pt
dc.subject Quelóide pt
dc.title Keloid heterograft in the hamster (Mesocricetus auratus) cheek pouch en
dc.title.alternative Heteroenxerto de quelóide na bolsa jugal do hamster (Mesocricetus auratus) pt
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation UNIFESP-EPM Post-Graduation Program
dc.description.affiliation UNIFESP-EPM Department of Surgery
dc.description.affiliation São Paulo University (USP) Immunology Department
dc.description.affiliation UNIFESP-EPM Department of Veterinary Medicine and Zootechnology
dc.description.affiliation UNIFESP-EPM Post-Graduation Program of Plastic Surgery
dc.description.affiliationUnifesp UNIFESP, EPM, Post-Graduation Program
dc.description.affiliationUnifesp UNIFESP, EPM Department of Surgery
dc.description.affiliationUnifesp UNIFESP, EPM Department of Veterinary Medicine and Zootechnology
dc.description.affiliationUnifesp UNIFESP, EPM Post-Graduation Program of Plastic Surgery
dc.identifier.file S0102-86502005000300003.pdf
dc.identifier.scielo S0102-86502005000300003
dc.identifier.doi 10.1590/S0102-86502005000300003
dc.description.source SciELO



File

Name: S0102-86502005000300003.pdf
Size: 535.5Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account