RECEPTORS for ANDROGEN-BINDING PROTEINS - INTERNALIZATION and INTRACELLULAR SIGNALING

RECEPTORS for ANDROGEN-BINDING PROTEINS - INTERNALIZATION and INTRACELLULAR SIGNALING

Autor Porto, C. S. Google Scholar
Lazari, MFM Google Scholar
Abreu, L. C. Google Scholar
Bardin, C. W. Google Scholar
Gunsalus, G. L. Google Scholar
Instituição POPULAT COUNCIL
Universidade Federal de São Paulo (UNIFESP)
Resumo In plasma, most steroid hormones are bound and transported by the specific binding protein, testosterone-estradiol-binding globulin (TeBG). for years, it was believed that the only function of this protein was to regulate the concentration of free steroids in plasma. However, a number of reports have provided evidence for the presence of specific TeBG receptors on plasma membranes. Furthermore, the interaction of TeBG with its receptor was shown to be inhibited when steroids are bound to TeBG, suggesting that TeBG is an allosteric protein. the purpose of this manuscript is to review the evidence that androgen-binding proteins bind to membrane receptors, and, in some cells, this binding stimulates cAMP accumulation, and transfer TeBG/ABP into tissue as a consequence of receptor mediated endocytosis. Recent studies from our laboratories have demonstrated binding and uptake of TeBG by MCF-7 breast cancer cells. the interaction of unligated rabbit TeBG with membranes from MCF-7 cells resulted in a time and concentration-dependent increase in adenylate cyclase activity. the TeBG alone also had a reproducible effect on intact cells by increasing cAMP accumulation by 30-35%. the addition of DHT to cells, after TeBG has been allowed to bind, resulted in increases in cAMP of greater than 4-fold. This effect was not blocked by antiandrogens. These data support the hypothesis that extracellular SHBG is a regulator of cellular function through a membrane receptor that is coupled to adenylate cyclase.
Idioma Inglês
Data de publicação 1995-06-01
Publicado em Journal of Steroid Biochemistry and Molecular Biology. Oxford: Pergamon-Elsevier B.V., v. 53, n. 1-6, p. 561-565, 1995.
ISSN 0960-0760 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 561-565
Fonte http://dx.doi.org/10.1016/0960-0760(95)00111-C
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1995RL31400079
Endereço permanente http://repositorio.unifesp.br/handle/11600/25502

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