ORGANIZATION and EXPRESSION of the GENE ENCODING AN IMMUNODOMINANT REPETITIVE ANTIGEN ASSOCIATED TO the CYTOSKELETON of TRYPANOSOMA-CRUZI

ORGANIZATION and EXPRESSION of the GENE ENCODING AN IMMUNODOMINANT REPETITIVE ANTIGEN ASSOCIATED TO the CYTOSKELETON of TRYPANOSOMA-CRUZI

Autor Cotrim, P. C. Google Scholar
Paranhosbaccala, G. Google Scholar
Santos, M. R. Google Scholar
Mortensen, C. Google Scholar
Cano, M. I. Google Scholar
Jolivet, M. Google Scholar
Camargo, M. E. Google Scholar
Mortara, R. A. Google Scholar
Dasilveira, J. F. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
BIOMERIEUX SA
Universidade de São Paulo (USP)
Resumo We have studied the genomic organization and expression of the gene encoding a high molecular mass (300 kDa) repetitive antigen associated with the cytoskeleton of Trypanosoma cruzi. Protease digestion of the native protein, restriction analysis of genomic DNA and sequencing of genomic and cDNA clones indicated that most of the protein is built up by tandemly arranged, nearly identical repeats of 68 amino acids. the gene size was estimated to be approx. 9.4 kb based on the sizes of the transcript and the native protein. the nucleotide sequence conservation among the repeats indicates that selective sequence homogenization, presumably through gene conversion, maintained the amino-acid sequence conservation. Two duplicated allelic forms of this gene were mapped in fragments of about 20 kb. in some strains an additional allele was located in a fragment of 9.4 kb. Our results suggest that this repetitive antigen is a structural protein which could be involved in the attachment of the flagellum to the cell body.
Palavra-chave TRYPANOSOMA CRUZI
IMMUNODOMINANT REPETITIVE ANTIGEN
CYTOSKELETON-ASSOCIATED PROTEIN
GENE ORGANIZATION
TANDEM REPEATS
GENE CONVERSION
Idioma Inglês
Data de publicação 1995-04-01
Publicado em Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 71, n. 1, p. 89-98, 1995.
ISSN 0166-6851 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 89-98
Fonte http://dx.doi.org/10.1016/0166-6851(95)00036-Z
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1995QZ09900008
Endereço permanente http://repositorio.unifesp.br/handle/11600/25482

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