PLASMA KALLIKREIN CLEARANCE BY the LIVER of CHRONIC CARBON TETRACHLORIDE-TREATED RATS

PLASMA KALLIKREIN CLEARANCE BY the LIVER of CHRONIC CARBON TETRACHLORIDE-TREATED RATS

Author Toledo, C. F. Google Scholar
Kouyoumdjian, M. Google Scholar
Lanzoni, V. P. Google Scholar
Borges, D. R. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We have previously reported that the endocytosis of rat plasma kallikrein (RPK) by hepatocytes is a calcium-independent and beta-galactoside-dependent mechanism. We now report the clearance of RPK by the liver of four groups of rats: normal, inflamed (48 h ex-turpentine) and two groups chronically treated with CCl4 (52 mg/kg per week, intragastrically, for 9-12 weeks). Each liver was isolated, exsanguinated and perfused at 37 degrees C with 30 mL of BSA-Krebs-Henseleit-bicarbonate medium containing 10 nmol/L RPK. Although all rats received the same mild CCl4 treatment, the liver histology showed that they evolved either to severe hepatitis (serum alanine aminotransferase [ALT] 4852 +/- 885 U/L, parenchymatous necrosis in the perivenous region) or to compensated cirrhosis (serum ALT 209 +/- 42 U/L, vigorous fibrous encircling regeneration nodules); neither jaundice nor ascites was noted. the results show that serum albumin was not altered among the groups and that: the acute-phase response by itself (inflamed group) increased RPK clearance rate (3.01 +/- 0.59 mL/min) as compared with the normal group (1.85 +/- 0.14 mL/min); the CCl4 treatment, although induced an acute-phase response, decreased (P < 0.01) RPK clearance rates (0.80 +/- 0.11 mL/min hepatitis group and 0.98 +/- 0.10 mL/min cirrhosis group). These findings suggest that the hepatic clearance rate of plasma kallikrein is an early indicator of liver injury.
Keywords ACUTE-PHASE
CIRRHOSIS
ENDOCYTOSIS
KALLIKREIN
LIVER
LIVER INJURY
TETRACHLORIDE-TREATED
TOXIC-HEPATITIS
Language English
Date 1995-03-01
Published in Journal of Gastroenterology and Hepatology. Carlton: Blackwell Science Publ Austr, v. 10, n. 2, p. 165-168, 1995.
ISSN 0815-9319 (Sherpa/Romeo, impact factor)
Publisher Blackwell Science Publ Austr
Extent 165-168
Origin http://dx.doi.org/10.1111/j.1440-1746.1995.tb01072.x
Access rights Closed access
Type Article
Web of Science ID WOS:A1995QN28600009
URI http://repositorio.unifesp.br/handle/11600/25474

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