COLLAGENASE-INDUCED EDEMA in the RAT PAW and the KININ SYSTEM

COLLAGENASE-INDUCED EDEMA in the RAT PAW and the KININ SYSTEM

Autor Pinto, JCS Google Scholar
Remaclevolon, G. Google Scholar
Sampaio, CAM Google Scholar
Damas, J. Google Scholar
Instituição UNIV LIEGE
Universidade Federal de São Paulo (UNIFESP)
Resumo Collagenase (100 mu g) induced a large plasma extravasation, during the first 15 min after its injection in rat paw, associated with the rapid development of oedema which subsided after 6 h. the extent of the oedema was similar in normal and kininogen-deficient rats. the swelling induced in normal rats was reduced by HOE 140 (D-Arg[Hyp(3),Thi(5),D-Tic(7),Oic(8)]bradykinin), a bradykinin B-2 receptor antagonist, and by three serine protease inhibitors, soybean trypsin inhibitor (SBTI), Leucaena leucocephala trypsin inhibitor 1 (LLTI-1) and Leucaena leucocephala trypsin inhibitor 2 (LLTI-2). These agents had no effect on the oedema induced in kininogen-deficient rats. the swelling was also reduced by methysergide, indomethacin, ketoprofen and methylprednisolone. It was increased by heparin, but it was not modified by mepyramine, WEB 2086 (3-[4-(2-chlorophenyl)-9 methyl-6H-thieno[3,2-f][1,2,4]-triazolo-[4,3-a][1,4]-diazepine-2-yl]-1-(4-morpholinyl)-1-propanone) and N-G-nitro-L-arginine. in vitro, collagenase did not release kinins from rat plasma or from purified T-kininogen. LLTI-1 and LLTI-2 did not inhibit collagenase activity for one of its specific substrates. Kinins are thus involved in the development of collagenase oedema in normal rats. Their generation would be indirect following changes in matrix proteins in extravascular spaces. Nevertheless, kinins are not the decisive mediators of the swelling. Serotonin, possibly released from platelets, and prostanoids participate in the inflammatory process.
Palavra-chave COLLAGENASE
INFLAMMATION
KININ
NITRIC OXIDE (NO)
SERINE PROTEASE INHIBITOR
PLATELET
Idioma Inglês
Data de publicação 1995-02-14
Publicado em European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 274, n. 1-3, p. 101-107, 1995.
ISSN 0014-2999 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 101-107
Fonte http://dx.doi.org/10.1016/0014-2999(94)00723-K
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1995QH20000014
Endereço permanente http://repositorio.unifesp.br/handle/11600/25464

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