DISCREPANCIES BETWEEN MIC and MLC VALUES of AMPHOTERICIN-B AGAINST ISOLATES of ASPERGILLUS SPECIES

DISCREPANCIES BETWEEN MIC and MLC VALUES of AMPHOTERICIN-B AGAINST ISOLATES of ASPERGILLUS SPECIES

Author Colombo, A. L. Google Scholar
Mcgough, D. A. Google Scholar
Rinaldi, M. G. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNIV TEXAS
Abstract There is little information addressing the phenomena of discrepancy between minimal inhibitory concentrations (MIG) and minimal lethal concentrations (MLC) values of amphotericin B (AMB) to clinical isolates of fungi. This study assessed in vitro activity of AMB against 70 clinical isolates of aspergilli: 30 strains of Aspergillus fumigatus, 20 strains of Aspergillus flavus and 20 strains of Aspergillus niger. Susceptibility tests were accomplished using a macro broth dilution procedure, with special emphasis on ascertainment of MLCs. AMB exhibited low MIC values against all clinical isolates. While we did not identify any AMB resistant isolates among 70 Aspergillus spp. studied as judged by MIC levels, analysis of the data demonstrated a clear discrepancy between the MIC and MLC levels of AMB obtained against clinical isolates of Aspergillus spp. the MLC values of AMB were significantly higher than the MIC values with MIC 50 and MIC 90 of 0.29 and 0.5 mu g/ml, respectively, at the second reading time, and MLC 50 and MLC 90 of 2.31 and 9.24 mu g/ml respectively (p < 0.001). Additionally, minimal lethal concentrations in 36/70 (51%) of aspergillal isolates studied produced drug concentrations above those which can usually be sustained in patient plasma or tissue.
Keywords AMPHOTERICIN B
ASPERGILLUS SP
SUSCEPTIBILITY TESTING
Language English
Date 1994-12-01
Published in Mycopathologia. Dordrecht: Kluwer Academic Publ, v. 128, n. 3, p. 129-133, 1994.
ISSN 0301-486X (Sherpa/Romeo, impact factor)
Publisher Kluwer Academic Publ
Extent 129-133
Origin http://dx.doi.org/10.1007/BF01138472
Access rights Closed access
Type Article
Web of Science ID WOS:A1994QX95200001
URI http://repositorio.unifesp.br/handle/11600/25450

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