GENES-CODING for SHIGA-LIKE TOXINS in BOVINE VEROTOXIN-PRODUCING ESCHERICHIA-COLI (VTEC) STRAINS BELONGING TO DIFFERENT O-K-H SEROTYPES

GENES-CODING for SHIGA-LIKE TOXINS in BOVINE VEROTOXIN-PRODUCING ESCHERICHIA-COLI (VTEC) STRAINS BELONGING TO DIFFERENT O-K-H SEROTYPES

Autor Blanco, M. Google Scholar
Blanco, J. Google Scholar
Blanco, J. E. Google Scholar
Gonzalez, E. A. Google Scholar
Gomes, TAT Google Scholar
Zerbini, L. F. Google Scholar
Yano, T. Google Scholar
Decastro, AFP Google Scholar
Instituição UNIV SANTIAGO
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Resumo Forty-six verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrhoeic and healthy calves in Spain were examined for DNA sequences homologous to genes for verotoxins (VT1 and VT2) and enterotoxins (LT-I, LT-II, STaH, STaP and STb). Hybridisation showed that 26 (57%) of VTEC strains carried VT1 genes, 13 (28%) possessed VT2 genes, and 7 (15%) carried both VT1 and VT2 genes. No VTEC strains hybridised with DNA probes for enterotoxins. A correlation was found between the serotype and type of VT produced. Thus, all strains of serotypes 026:K-:H11 (13 strains), 0103:K-:H2 (3 strains) and 0128:K?:H- (4 strains) hybridised with the VT1 probe only, whereas all strains of serotypes 03:K-:H4 (3 strains) and 0113:K-:H21 (4 strains) were positive with the VT2 probe only. By contrast, 081:K?:H28 (2 strains) and O157:K-:H- (2 strains) strains hybridised with both VT1 and VT2 probes. One strain of serotype O157:K-:H7 was VT2 positive.
Palavra-chave ESCHERICHIA COLI, GENETICS
DNA PROBE
PATHOGENICITY, BACTERIA
TOXIN
VT GENES
Idioma Inglês
Data de publicação 1994-11-01
Publicado em Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 42, n. 2-3, p. 105-110, 1994.
ISSN 0378-1135 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 105-110
Fonte http://dx.doi.org/10.1016/0378-1135(94)90010-8
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1994PV44600002
Endereço permanente http://repositorio.unifesp.br/handle/11600/25443

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