NOVEL ANTIGENIC DETERMINANTS FROM PEPTIDORHAMNOMANNANS of SPOROTHRIX-SCHENCKII

NOVEL ANTIGENIC DETERMINANTS FROM PEPTIDORHAMNOMANNANS of SPOROTHRIX-SCHENCKII

Autor Alver, L. L. Google Scholar
Travassos, L. R. Google Scholar
Previato, J. O. Google Scholar
Mendoncapreviato, L. Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Universidade do Estado do Rio de Janeiro (UERJ)
Universidade Federal de São Paulo (UNIFESP)
Resumo Antisera were raised in rabbits against acetone-dried yeast-like and mycelium forms of Sporothrix schenckii. These antisera were tested for immunoprecipitation of peptido-rhamnomannans isolated from both cell types. Both antisera reacted strongly with S.schenckii peptidorhamnomannans, but the reactions were weak with beta-eliminated peptidopolysaccharides. These antisera did not recognize the Saccharomyces cerevisiae mannoprotein, and reacted poorly with Ceratocystis (Ophiostoma) stenoceras cell wall glycopeptides. Since beta-eliminated glycopeptides were poorly reactive, we investigated the activity of O-glycosidically linked oligosaccharides which were liberated from the peptidorhamnomannans by mild alkaline hydrolysis, using a hapten inhibition test. the rates of inhibition showed that the immunodominant epitopes in O-linked tetra- and pentasaccharides had the following novel structures: alpha-L-Rhap (1-->4)-alpha-D-GlcpA and alpha-L-Rhap(1-->4)-[alpha-L-Rhap(1-->2)]alpha-D-GlcpA. Results suggest that peptidorhamnomannans or synthetic antigens bearing these epitopes should be used for the specific detection of anti-S.schenckii antibodies rather than the long-chain N-linked polysaccharides.
Palavra-chave CELL WALL
IMMUNOCHEMISTRY
PATHOGENIC DIMORPHIC FUNGUS
PEPTIDOPOLYSACCHARIDE
S-SCHENCKII
Idioma Inglês
Data de publicação 1994-06-01
Publicado em Glycobiology. Oxford: Oxford Univ Press United Kingdom, v. 4, n. 3, p. 281-288, 1994.
ISSN 0959-6658 (Sherpa/Romeo, fator de impacto)
Publicador Oxford Univ Press United Kingdom
Extensão 281-288
Fonte http://dx.doi.org/10.1093/glycob/4.3.281
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1994NV78100006
Endereço permanente http://repositorio.unifesp.br/handle/11600/25414

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