DECREASED DENSITY of BINDING-SITES for the CA2+ CHANNEL ANTAGONIST [H-3] ISRADIPINE AFTER DENERVATION of RAT VAS-DEFERENS

DECREASED DENSITY of BINDING-SITES for the CA2+ CHANNEL ANTAGONIST [H-3] ISRADIPINE AFTER DENERVATION of RAT VAS-DEFERENS

Autor Jurkiewicz, A. Google Scholar
Lafayette, S. S. Google Scholar
Nunes, S. H. Google Scholar
Martini, L. C. Google Scholar
Docarmo, LGD Google Scholar
Wanderley, A. G. Google Scholar
Jurkiewicz, N. H. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Radioligand binding assays were performed with the selective antagonist of dihydropyridine-sensitive Ca2+ channels [H-3]PN200-110 (isradipine) in rat vas deferens, before and 7 days after denervation, and data were compared with those obtained for K+-induced contractions, which are Ca2+-dependent. the density (B-max) of dihydropyridine binding sites was decreased to almost one-third of its normal value after denervation. the respective affinity (K-D) was not significantly changed. in addition, it was observed that the K+-induced tonic contraction, which corresponded to 55 +/- 2% of the respective phasic contraction, was decreased to 41 +/- 3% after: denervation. It is assumed that the decreased density of Ca2+ channels causes a decrease in K+-induced influx of Ca2+ and consequently of the corresponding tonic contraction. These results indicate that autonomic innervation can regulate the density of dihydropyridine-sensitive Ca2+ channels in the rat vas deferens.
Palavra-chave VAS DEFERENS
DENERVATION
CA2+ CHANNEL
DIHYDROPYRIDINE
ISRADIPINE
Idioma Inglês
Data de publicação 1994-05-02
Publicado em European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 256, n. 3, p. 329-333, 1994.
ISSN 0014-2999 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 329-333
Fonte http://dx.doi.org/10.1016/0014-2999(94)90559-2
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1994NK58000012
Endereço permanente http://repositorio.unifesp.br/handle/11600/25407

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