STRUCTURAL REQUIREMENTS of BIOACTIVE PEPTIDES for INTERACTION WITH ENDOPEPTIDASE-22.19

STRUCTURAL REQUIREMENTS of BIOACTIVE PEPTIDES for INTERACTION WITH ENDOPEPTIDASE-22.19

Autor Camargo, ACM Google Scholar
Gomes, M. D. Google Scholar
Toffoletto, O. Google Scholar
Ribeiro, MJF Google Scholar
Ferro, E. S. Google Scholar
Fernandes, B. L. Google Scholar
Suzuki, K. Google Scholar
Sasaki, Y. Google Scholar
Juliano, L. Google Scholar
Instituição Universidade de São Paulo (USP)
TOKOHU COLL PHARM
Universidade Federal de São Paulo (UNIFESP)
Resumo A series of biologically active peptides and related compounds (opioid peptides, neurotensin, and bradykinin) were used as substrates or competitive inhibitors to study the structural requirements for peptide interaction with endopeptidase 22.19. the kinetics of hydrolysis of these peptides indicated that, in contrast to other proteases, the substrate specificity of endopeptidase 22.19 is not determined by the amino acids flanking the sensitive bonds of the substrates. the competition between bioactive peptide analogues and the quenched fluorescence substrate of endopeptidase 22.19 indicated that their length and their flexibility may be the dominant factors to explain their binding specificities. These peculiar features of endopeptidase 22.19 may be of importance to understand the physiological processes of conversion and inactivation of biologically active peptides.
Idioma Inglês
Data de publicação 1994-04-01
Publicado em Neuropeptides. Edinburgh: Churchill Livingstone, v. 26, n. 4, p. 281-287, 1994.
ISSN 0143-4179 (Sherpa/Romeo, fator de impacto)
Publicador Churchill Livingstone
Extensão 281-287
Fonte http://dx.doi.org/10.1016/0143-4179(94)90083-3
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1994NE63000011
Endereço permanente http://repositorio.unifesp.br/handle/11600/25395

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