DETECTION of ANTIBODIES in SERA FROM CHAGAS-DISEASE PATIENTS USING A TRYPANOSOMA-CRUZI IMMUNODOMINANT RECOMBINANT ANTIGEN

DETECTION of ANTIBODIES in SERA FROM CHAGAS-DISEASE PATIENTS USING A TRYPANOSOMA-CRUZI IMMUNODOMINANT RECOMBINANT ANTIGEN

Autor Paranhosbacalla, G. S. Google Scholar
Santos, MRM Google Scholar
Cotrim, P. Google Scholar
Rassi, A. Google Scholar
Jolivet, M. Google Scholar
Camargo, M. E. Google Scholar
Dasilveira, J. F. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
BIOMERIEUX
BIOLAB DIAGNOST
Universidade Federal de Goiás (UFG)
Universidade de São Paulo (USP)
Resumo A Trypanosoma cruzi DNA fragment encoding an immunodominant repetitive antigen (H49) was subcloned into a protein purification and expressed system. Purified H49 peptide reacted specifically in an enzyme-linked immunosorbent assay (ELISA) with sera from T. cruzi-infected patients, but not with sera from patients with other parasitic diseases such as leishmaniasis and T. rangeli-infection. the H49 recombinant ELISA was able to detect specific antibodies in 84% of chronic chagasic serum samples tested. One of the major advantage of the recombinant ELISA for serodiagnosis of chronic Chagas' disease resides in its high specificity (100%). Our data suggest that recombinant peptides could provide a practical basis for specific diagnosis tests for Chagas' disease.
Palavra-chave CHAGAS DISEASE
RECOMBINANT ANTIGEN
IMMUNODOMINANT REPETITIVE ANTIGEN
ELISA
SERODIAGNOSIS
Idioma Inglês
Data de publicação 1994-03-01
Publicado em Parasite Immunology. Oxford: Blackwell Science Ltd, v. 16, n. 3, p. 165-169, 1994.
ISSN 0141-9838 (Sherpa/Romeo, fator de impacto)
Publicador Blackwell Science Ltd
Extensão 165-169
Fonte http://dx.doi.org/10.1111/j.1365-3024.1994.tb00336.x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1994NC54300006
Endereço permanente http://repositorio.unifesp.br/handle/11600/25389

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