DISTRIBUTION of ALPHA-GALACTOSYL-CONTAINING EPITOPES ON TRYPANOSOMA-CRUZI TRYPOMASTIGOTE and AMASTIGOTE FORMS FROM INFECTED VERO CELLS DETECTED BY CHAGASIC ANTIBODIES

DISTRIBUTION of ALPHA-GALACTOSYL-CONTAINING EPITOPES ON TRYPANOSOMA-CRUZI TRYPOMASTIGOTE and AMASTIGOTE FORMS FROM INFECTED VERO CELLS DETECTED BY CHAGASIC ANTIBODIES

Autor Soutopadron, T. Google Scholar
Almeida, I. C. Google Scholar
Desouza, W. Google Scholar
Travassos, L. R. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Resumo Reactivity of different Trypanosoma cruzi developmental forms with purified Chagasic anti-alpha-galactosyl antibodies (anti-Gal) was studied using epimastigotes from axenic cultures, trypomastigotes and amastigotes from infected Vero cell cultures, and an immunogold labeling method as observed by electron microscopy. Epimastigotes were poorly labeled, whereas extracellular trypomastigotes and amastigotes bound heterogeneously to the antibody with many cells being intensely labeled at the cell surface, including the membrane lining the cell body, the flagellum and the flagellar pocket. Parasites with poor labeling at the cell surface generally had several gold particles within the cell, mostly in cytoplasmic vacuoles. the Golgi complex of trypomastigotes was strongly labeled. Intracellular parasites were labeled at the parasite cell surface or within vacuolar structures. the expression in T. cruzi-infected Vero cells of alpha-galactosyl antigenic structures acquired from the parasite was shown by moderate labeling viith Chagasic anti-Gal of the membrane lining parasite-free outward cell projections. the reactivity with purified anti-Gal from healthy individuals at the same concentrations of Chagasic anti-Gal was poor, with gold particles appearing in the nucleus and cytoplasm but not at the cell surface. It paralleled the labeling with Bandeireae simplicifolia IB-3 lectin. the results provide a basis for autoimmune reactions involving anti-Gal from chronic Chagasic patients.
Palavra-chave ANTI-ALPHA-GALACTOSYL ANTIBODIES
CHAGAS DISEASE
IMMONUGOLD LABELING
Idioma Inglês
Data de publicação 1994-01-01
Publicado em Journal of Eukaryotic Microbiology. Lawrence: Soc Protozoologists, v. 41, n. 1, p. 47-54, 1994.
ISSN 1066-5234 (Sherpa/Romeo, fator de impacto)
Publicador Soc Protozoologists
Extensão 47-54
Fonte http://dx.doi.org/10.1111/j.1550-7408.1994.tb05933.x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1994MW33700008
Endereço permanente http://repositorio.unifesp.br/handle/11600/25381

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta