CIRCUIT MECHANISMS of SEIZURES in the PILOCARPINE MODEL of CHRONIC EPILEPSY - CELL LOSS and MOSSY FIBER SPROUTING

CIRCUIT MECHANISMS of SEIZURES in the PILOCARPINE MODEL of CHRONIC EPILEPSY - CELL LOSS and MOSSY FIBER SPROUTING

Autor Mello, LEAM Google Scholar
Cavalheiro, E. A. Google Scholar
Tan, A. M. Google Scholar
Kupfer, W. R. Google Scholar
Pretorius, J. K. Google Scholar
Babb, T. L. Google Scholar
Finch, D. M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
UNIV CALIF LOS ANGELES
Resumo We used the pilocarpine model of chronic spontaneous recurrent seizures to evaluate the time course of supragranular dentate sprouting and to assess the relation between several changes that occur in epileptic tissue with different behavioral manifestations of this experimental model of temporal lobe epilepsy. Pilocarpine-induced status epilepticus (SE) invariably led to cell loss in the hilus of the dentate gyrus (DG) and to spontaneous recurrent seizures. Cell loss was often also noted in the DG and in hippocampal subfields CA1 and CA3. the seizures began to appear at a mean of 15 days after SE induction (silent period), recurred at variable frequencies for each animal, and lasted for as long as the animals were allowed to survive (325 days). the granule cell layer of the DG was dispersed in epileptic animals, and neo-Timm stains showed supra- and intragranular messy fiber sprouting. Supragranular messy fiber sprouting and dentate granule cell dispersion began to appear early after SE (as early as 4 and 9 days, respectively) and reached a plateau by 100 days. Animals with a greater degree of cell loss in hippocampal field CA3 showed later onset of chronic epilepsy (r = 0.83, p < 0.0005), suggesting that CA3 represents one of the routes for seizure spread. These results demonstrate that the pilocarpine model of chronic seizures replicates several of the features of human temporal lobe epilepsy (hippocampal cell loss, supra- and intragranular messy fiber sprouting, dentate granule cell dispersion, spontaneous recurrent seizures) and that it may be a useful model for studying this human condition. the results also suggest that even though a certain amount of cell loss in specific areas may be essential for chronic seizures to occur, excessive cell loss may hinder epileptogenesis.
Palavra-chave HIPPOCAMPUS
NEUROPATHOLOGY
EPILEPTOGENESIS
BEHAVIOR
STATUS EPILEPTICUS
NEUROLOGIC MODELS
CONVULSANTS
PILOCARPINE
Idioma Inglês
Data de publicação 1993-11-01
Publicado em Epilepsia. Philadelphia: Lippincott-raven Publ, v. 34, n. 6, p. 985-995, 1993.
ISSN 0013-9580 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott-raven Publ
Extensão 985-995
Fonte http://dx.doi.org/10.1111/j.1528-1157.1993.tb02123.x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1993MM46000003
Endereço permanente http://repositorio.unifesp.br/handle/11600/25363

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