MUCIN-LIKE GLYCOPROTEINS LINKED TO the MEMBRANE BY GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ARE the MAJOR ACCEPTORS of SIALIC-ACID in A REACTION CATALYZED BY TRANS-SIALIDASE in METACYCLIC FORMS of TRYPANOSOMA-CRUZI

MUCIN-LIKE GLYCOPROTEINS LINKED TO the MEMBRANE BY GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ARE the MAJOR ACCEPTORS of SIALIC-ACID in A REACTION CATALYZED BY TRANS-SIALIDASE in METACYCLIC FORMS of TRYPANOSOMA-CRUZI

Autor Schenkman, S. Google Scholar
Ferguson, MAJ Google Scholar
Heise, N. Google Scholar
Dealmeida, MLC Google Scholar
Mortara, R. A. Google Scholar
Yoshida, N. Google Scholar
Instituição UNIV DUNDEE
Universidade Federal de São Paulo (UNIFESP)
Resumo We have previously shown that 35- and 50-kDa glycoconjugates of cultured metacyclic trypomastigotes participate in the attachment of parasites to mammalian cells. Here we show that when metacyclic trypomastigotes are incubated with [H-3]sialyllactose, most of the sialic acid is transferred to these 35/50-kDa molecules in a reaction catalyzed by a parasite transsialidase. the sialic acid is incorporated in oligosaccharides of about 10 glucose units in size that are released from the glycoconjugate by mild alkaline hydrolysis. Compositional analysis reveals that the 35/50-kDa molecules are highly glycosylated proteins rich in threonine, galactose, N-acetyl-glucosamine and sialic acid. These glycoproteins can be labeled in vivo with [H-3]palmitate, and the labeled fatty acid is released by glycosylphosphatidylinositol specific phospholipases C. This result, associated with the fact that they contain mannose, ethanolamine, myo-inositol, and lipid, indicate that these glycoproteins are anchored to the membrane by glycosylphosphatidylinositol. During cell invasion, these molecules appear to be capped and locally released by the parasite.
Palavra-chave TRYPANOSOMA-CRUZI
SIALIC ACID
TRANS-SIALIDASE
CELL INVASION
GLYCOSYLPHOSPHATIDYLINOSITOL
Idioma Inglês
Data de publicação 1993-06-01
Publicado em Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 59, n. 2, p. 293-303, 1993.
ISSN 0166-6851 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 293-303
Fonte http://dx.doi.org/10.1016/0166-6851(93)90227-O
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1993LE84900012
Endereço permanente http://repositorio.unifesp.br/handle/11600/25331

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