ABSENCE of CELL-WALL CHITIN in SACCHAROMYCES-CEREVISIAE LEADS TO RESISTANCE TO KLUYVEROMYCES-LACTIS KILLER TOXIN

ABSENCE of CELL-WALL CHITIN in SACCHAROMYCES-CEREVISIAE LEADS TO RESISTANCE TO KLUYVEROMYCES-LACTIS KILLER TOXIN

Author Takita, M. A. Google Scholar
Castilhovalavicius, B. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Kluyveromyces lactis killer toxin causes sensitive strains of a variety of yeasts to arrest at the GI stage of the cell cycle, and to lose viability. We describe here the isolation and characterization of a class of recessive mutations in Saccharomyces cerevisiae that leads to toxin resistance and a temperature-sensitive phenotype. These mutant cells arrest growth at 37-degrees-C with a characteristic phenotype of elongated buds. Cloning of the gene complementing these defects revealed it to be CAL1, coding for chitin synthase 3 activity. Calcofluor staining of the mutant cells indicated that chitin is absent both at 23-degrees-C and 37-degrees-C. Given that the CAL1 activity is responsible for the synthesis of most of chitin in yeast cells, and that in its absence the cells are viable but resistant to the killer toxin, our results strongly suggest that chitin might represent the receptor for this killer toxin.
Keywords SACCHAROMYCES-CEREVISIAE
KLUYVEROMYCES-LACTIS
KILLER TOXIN
FUNGAL CHITIN
CELL WALL MUTANTS
Language English
Date 1993-06-01
Published in Yeast. W Sussex: John Wiley & Sons Ltd, v. 9, n. 6, p. 589-598, 1993.
ISSN 0749-503X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 589-598
Origin http://dx.doi.org/10.1002/yea.320090605
Access rights Closed access
Type Article
Web of Science ID WOS:A1993LJ56600004
URI http://repositorio.unifesp.br/handle/11600/25329

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