COMMON EPITOPES in the CIRCUMSPOROZOITE PROTEINS of PLASMODIUM-BERGHEI and PLASMODIUM-GALLINACEUM IDENTIFIED BY MONOCLONAL-ANTIBODIES TO the P-GALLINACEUM CIRCUMSPOROZOITE PROTEIN

COMMON EPITOPES in the CIRCUMSPOROZOITE PROTEINS of PLASMODIUM-BERGHEI and PLASMODIUM-GALLINACEUM IDENTIFIED BY MONOCLONAL-ANTIBODIES TO the P-GALLINACEUM CIRCUMSPOROZOITE PROTEIN

Autor Rocha, EMM Google Scholar
Hollingdale, M. R. Google Scholar
Sina, B. Google Scholar
Leland, P. Google Scholar
Lopes, J. D. Google Scholar
Krettli, A. U. Google Scholar
Instituição BIOMED RES INST
Universidade Federal de São Paulo (UNIFESP)
FIOCRUZ MS
Resumo Monoclonal antibodies that react with the circumsporozoite protein of the avian malaria Plasmodium gallinaceum sporozoites also reacted with circumsporozoite protein of the rodent malaria Plasmodium berghei. Two types of reactivity were identified: 1) two monoclonal antibodies reacted with P. berghei sporozoite protein by enzyme-linked immunosorbent assay, Western blot and indirect immunofluorescence antibody, 2) six other monoclonal antibodies reacted with P. berghei sporozoites by ELISA and Western blot only. We studied whether these differences could be explained by reactivity in enzyme-linked immunosorbent assay with different P. berghei circumsporozoite peptides. Although all P. gallinaceum monoclonal antibodies reacted with the P. berghei repeats, the first group reacted with a conserved peptide sequence, N1, whereas the second group did not. These results suggest that circumsporozoite proteins from P. gallinaceum and P. berghei share common epitopes. the biological significance of our finding is not yet clear. Indeed, the cross-reactive monoclonal antibodies giving a positive indirect immunofluorescence antibody with the P. berghei sporozoites only caused a borderline effect on the living P. berghei parasites in vitro as measured by inhibition of sporozoite infectivity.
Palavra-chave ANTISPOROZOITE MONOCLONAL ANTIBODIES
CIRCUMSPOROZOITE PROTEIN
PLASMODIUM-BERGHEI
PLASMODIUM-GALLINACEUM
Idioma Inglês
Data de publicação 1993-01-01
Publicado em Journal of Eukaryotic Microbiology. Lawrence: Soc Protozoologists, v. 40, n. 1, p. 61-63, 1993.
ISSN 1066-5234 (Sherpa/Romeo, fator de impacto)
Publicador Soc Protozoologists
Extensão 61-63
Fonte http://dx.doi.org/10.1111/j.1550-7408.1993.tb04882.x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1993KQ68800010
Endereço permanente http://repositorio.unifesp.br/handle/11600/25299

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