MOLECULAR EPIDEMIOLOGY and ANTIMICROBIAL SUSCEPTIBILITY TESTING - TESTING of QUINOLONE-RESISTANT STAPHYLOCOCCUS-AUREUS STRAINS ISOLATED in BRAZIL

MOLECULAR EPIDEMIOLOGY and ANTIMICROBIAL SUSCEPTIBILITY TESTING - TESTING of QUINOLONE-RESISTANT STAPHYLOCOCCUS-AUREUS STRAINS ISOLATED in BRAZIL

Autor Rodrigues, J. N. Google Scholar
Amaral, JLG Google Scholar
Leme, I. L. Google Scholar
Pignatari, A. Google Scholar
Wey, S. Google Scholar
Hollis, R. Google Scholar
Pfaller, M. A. Google Scholar
Jones, R. N. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
UNIV IOWA
Resumo An outbreak of 20 oxacillin-resistant Staphylococcus aureus (ORSA) bloodstream infections (BSIs) was detected in the intensive care unit (ICU) at Hospital São Paulo, Brazil. in a surveillance study, 10% of ICU personnel were defined as chronic nasal carriers of ORSA. Thirteen BSIs and five nasal isolates were available for phage typing, restriction endonuclease analysis of plasmid (REAP) and chromosomal DNA hybridized with P-32 rRNA gene probe. Susceptibility testing against select antimicrobial agents, including 11 quinolones, six glycopeptides, and five topical agents, was performed by broth microdilution and the disk diffusion tests. Ten of the 13 BSI isolates and four of the five nasal strains were oxacillin and quinolone resistant. the new fluoroquinolones CI-960 and WIN57273, the glycopeptides, and the topical agents ramoplanin, bacitracin, mupirocin, and novobiocin were most active. Fourteen strains, 12 BSI isolates, and three nasal isolates showed the same REAP profile. Moreover, the same REAP and chromosomal profile was detected in at least nine BSI isolates and in two nasal isolates. These strains were nontypable by phage typing. We concluded that nosocomial cross-transmission of a single, multiresistant strain of S. aureus occurred and that the epidemic reservoir was nasal carriage by ICU personnel.
Idioma Inglês
Data de publicação 1993-01-01
Publicado em Diagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 16, n. 1, p. 9-16, 1993.
ISSN 0732-8893 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 9-16
Fonte http://dx.doi.org/10.1016/0732-8893(93)90124-P
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1993KH47300002
Endereço permanente http://repositorio.unifesp.br/handle/11600/25297

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