GLYCOCONJUGATES of TRYPANOSOMA-CRUZI - A 74 KD ANTIGEN of TRYPOMASTIGOTES SPECIFICALLY REACTS WITH LYTIC ANTI-ALPHA-GALACTOSYL ANTIBODIES FROM PATIENTS WITH CHRONIC CHAGAS-DISEASE

GLYCOCONJUGATES of TRYPANOSOMA-CRUZI - A 74 KD ANTIGEN of TRYPOMASTIGOTES SPECIFICALLY REACTS WITH LYTIC ANTI-ALPHA-GALACTOSYL ANTIBODIES FROM PATIENTS WITH CHRONIC CHAGAS-DISEASE

Autor Almeida, I. C. Google Scholar
Krautz, G. M. Google Scholar
Krettli, A. U. Google Scholar
Travassos, L. R. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
FUNDACAO OSWALDO CRUZ
Resumo Protective, lytic antibodies are believed to be correlated with active Trypanosoma cruzi infection. in patients with chronic infection, antibodies lysing trypomastigote forms recognize chiefly alpha-galactosyl structures at the parasite surface. the target molecules on cell-derived trypomastigotes that react with anti-alpha-galactosyl antibodies (anti-Gal) from patients with chronic Chagas disease were investigated. Glycoconjugates were isolated from trypomastigotes and shown to absorb purified Chagasic (Ch) anti-Gal effectively as well as lytic antibodies from Ch sera. Active fractions were F2 (74 kD and 95.6 kD) and F3 (120-200 kD). A differential reactivity with antibodies from untreated Ch patients (trypanolytic) and from treated. presumably cured. individuals (not trypanolytic) was evident using F2 and F3 antigenic fractions. No cross-reactivity with heterologous sera (other infections) was observed. the F2 glycoconjugate (mostly 74 kD) can be used in the diagnosis of active Chagas infection, replacing the quantitative determination of complement-mediated lysis. With the present sample of patients' sera and normal human sera, it showed 100% sensitivity and specificity. (C) 1993 Wiley-Liss. Inc.
Palavra-chave AMERICAN TRYPANOSOMIASIS
ANTI-GAL ANTIBODY
IMMUNOLOGICAL DIAGNOSIS
DRUG-TREATED PATIENTS
TRYPANOLYSIS
CHEMOLUMINESCENCE
Idioma Inglês
Data de publicação 1993-01-01
Publicado em Journal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 7, n. 6, p. 307-316, 1993.
ISSN 0887-8013 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 307-316
Fonte http://dx.doi.org/10.1002/jcla.1860070603
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1993MD08500002
Endereço permanente http://repositorio.unifesp.br/handle/11600/25295

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