INDUCTION of AN IMMUNE-RESPONSE THROUGH the IDIOTYPIC NETWORK WITH MONOCLONAL ANTIIDIOTYPE ANTIBODIES in the CARCINOEMBRYONIC ANTIGEN SYSTEM

INDUCTION of AN IMMUNE-RESPONSE THROUGH the IDIOTYPIC NETWORK WITH MONOCLONAL ANTIIDIOTYPE ANTIBODIES in the CARCINOEMBRYONIC ANTIGEN SYSTEM

Autor Demoraes, J. Z. Google Scholar
Carneiro, CRW Google Scholar
Buchegger, F. Google Scholar
Mach, J. P. Google Scholar
Lopes, J. D. Google Scholar
Instituição LUDWIG INST CANC RES
UNIV LAUSANNE
Universidade Federal de São Paulo (UNIFESP)
Resumo Anti-idiotype antibodies can mimic the conformational epitopes of the original antigen and act as antigen substitutes for vaccination and/or serological purposes. To investigate this possibility concerning the tumor marker carcinoembryonic antigen (CEA), BALB/c mice were immunized with the previously described anti-CEA monoclonal antibody (MAb) 5.D11 (AB1). After cell fusion, 15 stable cloned cell lines secreting anti-Ids (AB2) were obtained. Selected MAbs gave various degrees of inhibition (up to 1 00%) of the binding of I-125-labeled CEA to MAb 5.Dl 1. Absence of reactivity of anti-Id MAbs with normal mouse IgG was first demonstrated by the fact that anti-Id MAbs were not absorbed by passage through a mouse IgG column, and second because they bound specifically to non-reduced MAb 5.D11 on Western blots. Anti-5.D11 MAbs did not inhibit binding to CEA of MAb 10.B9, another anti-CEA antibody obtained in the same fusion as 5.D11, or that of several anti-CEA MAbs reported in an international workshop, with the exception of two other anti-CEA MAbs, both directed against the GOLD IV epitope. When applied to an Id-anti-Id competitive radioimmunoassay, a sensitivity of 2 ng/ml of CEA was obtained, which is sufficient for monitoring circulating CEA in carcinoma patients. To verify that the anti-Id MAbs have the potential to be used as CEA vaccines, syngeneic BALB/c mice were immunized with these MAbs (AB2). Sera from immunized mice were demonstrated to contain AB3 antibodies recognizing the original antigen, CEA, both in enzyme immunoassay and by immunoperoxidase staining of human colon carcinoma. These results open the perspective of vaccination against colorectal carcinoma through the use of anti-idiotype antibodies as antigen substitutes.
Palavra-chave COLORECTAL CARCINOMA
CEA
IDIOTYPE
GOLD IV
TUMOR VACCINE
Idioma Inglês
Data de publicação 1992-11-01
Publicado em Journal of Cellular Biochemistry. Hoboken: Wiley-liss, v. 50, n. 3, p. 324-335, 1992.
ISSN 0730-2312 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 324-335
Fonte http://dx.doi.org/10.1002/jcb.240500313
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1992JW89900012
Endereço permanente http://repositorio.unifesp.br/handle/11600/25280

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