Increased density of alpha-adrenoceptors in vas-deferens of spontaneously hypertensive rats (SHR), indicated by functional and receptor-binding studies

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dc.contributor.author Caricati-Neto, Afonso [UNIFESP]
dc.contributor.author Sette, M.
dc.contributor.author Jurkiewicz, A.
dc.date.accessioned 2016-01-24T11:40:08Z
dc.date.available 2016-01-24T11:40:08Z
dc.date.issued 1992-07-21
dc.identifier http://dx.doi.org/10.1016/0014-2999(92)90146-U
dc.identifier.citation European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 218, n. 1, p. 51-58, 1992.
dc.identifier.issn 0014-2999
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/25266
dc.description.abstract Pharmacological parameters were determined from contractile responses mediated by alpha-adrenoceptors in vas deferens from spontaneously hypertensive rats (SHR) and corresponding normotensive controls, Wistar Kyoto rats (WKY), and compared with data obtained from radioligand binding assays. Contractile responses induced in longitudinal and circular muscle layers by the alpha-adrenoceptor agonist noradrenaline (NA) and by barium chloride were recorded as described previously. in both muscle layers the maximal effects induced by NA, but not by BaCl2, were significantly greater in SHR. As a consequence, the relative responsiveness ratio (rho) for the alpha-adrenoceptor was also larger for SHR than for WKY. NA-induced contractions of both muscle layers were competitively antagonized by indoramine. the pA2 values for indoramine and pD2 values for NA were the same in SHR and WKY, indicating that alpha-adrenoceptor affinity was not changed in SHR. Additionally, binding studies with thc alpha-adrenoceptor ligand [H-3]WB4101 revealed that B(max) values were greater in the vas deferens of SHR, whereas K(d) values were not significantly different from those of WKY controls. in summary, although differences could not be detected for affinity-related parameters, a greater density of alpha-adrenoceptors was shown for SHR in receptor binding studies and this was corroborated by functional studies. en
dc.format.extent 51-58
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof European Journal of Pharmacology
dc.rights Acesso restrito
dc.subject VAS DEFERENS en
dc.subject SMOOTH MUSCLE en
dc.subject ALPHA-ADRENOCEPTORS en
dc.subject SPONTANEOUSLY HYPERTENSIVE RATS (SHR) en
dc.title Increased density of alpha-adrenoceptors in vas-deferens of spontaneously hypertensive rats (SHR), indicated by functional and receptor-binding studies en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation ESCOLA PAULISTA MED SCH,DEPT PHARMACOL,CAIXA POSTAL 20372,BR-04034 São Paulo,BRAZIL
dc.description.affiliationUnifesp ESCOLA PAULISTA MED SCH,DEPT PHARMACOL,CAIXA POSTAL 20372,BR-04034 São Paulo,BRAZIL
dc.identifier.doi 10.1016/0014-2999(92)90146-U
dc.description.source Web of Science
dc.identifier.wos WOS:A1992JG59000007



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