ENDO-OLIGOPEPTIDASE-A, A PUTATIVE ENKEPHALIN-GENERATING ENZYME, in the VERTEBRATE RETINA

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dc.contributor.author Ferro, E. S.
dc.contributor.author Hamassaki, D. E.
dc.contributor.author Camargo, ACM
dc.contributor.author Britto, LRG
dc.date.accessioned 2016-01-24T11:40:05Z
dc.date.available 2016-01-24T11:40:05Z
dc.date.issued 1991-11-01
dc.identifier http://dx.doi.org/10.1111/j.1471-4159.1991.tb06363.x
dc.identifier.citation Journal of Neurochemistry. Philadelphia: Lippincott-raven Publ, v. 57, n. 5, p. 1643-1649, 1991.
dc.identifier.issn 0022-3042
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/25228
dc.description.abstract Endo-oligopeptidase A, EC 3.4.22.19, converts small enkephalin-containing peptides into the corresponding enkephalins in vitro. We investigated the presence of endooligopeptidase A in the retina and its possible colocalization with enkephalins in retinal neurons. the specific activity of endo-oligopeptidase A found in pigeon retinae (30.3 +/- 7.3 mU/mg, mean +/- standard deviation) was four times higher than in rabbit retinae (7.0 +/- 1.1 mU/mg). the enzyme activity was not modified by EDTA, but it was enhanced by dithiothreitol and inhibited by zinc and 5,5'-dithiobis(2-nitrobenzoic acid). Immunohistochemical experiments with a purified antiserum against rabbit endo-oligopeptidase A revealed labeled neurons in both the inner nuclear layer and the ganglion cell layer of pigeon and rabbit retinae. Double-labeling immunofluorescence experiments demonstrated that about 90% of neurons containing endo-oligopeptidase A-like immunoreactivity also contained [Leu5]-enkephalin-like immunoreactivity. These colocalization results may represent an important step toward the demonstration of the possible involvement of endo-oligopeptidase A in enkephalin generation in vivo. en
dc.format.extent 1643-1649
dc.language.iso eng
dc.publisher Lippincott-raven Publ
dc.relation.ispartof Journal of Neurochemistry
dc.rights Acesso restrito
dc.subject ENDO-OLIGOPEPTIDASE-A en
dc.subject ENDOPEPTIDASE 22.19 en
dc.subject ENKEPHALINS en
dc.subject OPIOID PEPTIDES en
dc.subject RETINAL NEUROPEPTIDES en
dc.title ENDO-OLIGOPEPTIDASE-A, A PUTATIVE ENKEPHALIN-GENERATING ENZYME, in the VERTEBRATE RETINA en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution São Paulo STATE UNIV
dc.description.affiliation ESCOLA PAULISTA MED SCH,DEPT BIOPHYS,BR-04023 São Paulo,BRAZIL
dc.description.affiliation São Paulo STATE UNIV,INST BIOMED SCI,DEPT PHYSIOL & BIOPHYS,BR-05508 São Paulo,BRAZIL
dc.description.affiliationUnifesp ESCOLA PAULISTA MED SCH,DEPT BIOPHYS,BR-04023 São Paulo,BRAZIL
dc.identifier.doi 10.1111/j.1471-4159.1991.tb06363.x
dc.description.source Web of Science
dc.identifier.wos WOS:A1991GL28800025



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