ENDO-OLIGOPEPTIDASE-A, A PUTATIVE ENKEPHALIN-GENERATING ENZYME, in the VERTEBRATE RETINA

ENDO-OLIGOPEPTIDASE-A, A PUTATIVE ENKEPHALIN-GENERATING ENZYME, in the VERTEBRATE RETINA

Autor Ferro, E. S. Google Scholar
Hamassaki, D. E. Google Scholar
Camargo, ACM Google Scholar
Britto, LRG Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
São Paulo STATE UNIV
Resumo Endo-oligopeptidase A, EC 3.4.22.19, converts small enkephalin-containing peptides into the corresponding enkephalins in vitro. We investigated the presence of endooligopeptidase A in the retina and its possible colocalization with enkephalins in retinal neurons. the specific activity of endo-oligopeptidase A found in pigeon retinae (30.3 +/- 7.3 mU/mg, mean +/- standard deviation) was four times higher than in rabbit retinae (7.0 +/- 1.1 mU/mg). the enzyme activity was not modified by EDTA, but it was enhanced by dithiothreitol and inhibited by zinc and 5,5'-dithiobis(2-nitrobenzoic acid). Immunohistochemical experiments with a purified antiserum against rabbit endo-oligopeptidase A revealed labeled neurons in both the inner nuclear layer and the ganglion cell layer of pigeon and rabbit retinae. Double-labeling immunofluorescence experiments demonstrated that about 90% of neurons containing endo-oligopeptidase A-like immunoreactivity also contained [Leu5]-enkephalin-like immunoreactivity. These colocalization results may represent an important step toward the demonstration of the possible involvement of endo-oligopeptidase A in enkephalin generation in vivo.
Palavra-chave ENDO-OLIGOPEPTIDASE-A
ENDOPEPTIDASE 22.19
ENKEPHALINS
OPIOID PEPTIDES
RETINAL NEUROPEPTIDES
Idioma Inglês
Data de publicação 1991-11-01
Publicado em Journal of Neurochemistry. Philadelphia: Lippincott-raven Publ, v. 57, n. 5, p. 1643-1649, 1991.
ISSN 0022-3042 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott-raven Publ
Extensão 1643-1649
Fonte http://dx.doi.org/10.1111/j.1471-4159.1991.tb06363.x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1991GL28800025
Endereço permanente http://repositorio.unifesp.br/handle/11600/25228

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