NMDA RECEPTOR-MEDIATED EXCITABILITY in DENDRITICALLY DEFORMED DENTATE GRANULE CELLS in PILOCARPINE-TREATED RATS

NMDA RECEPTOR-MEDIATED EXCITABILITY in DENDRITICALLY DEFORMED DENTATE GRANULE CELLS in PILOCARPINE-TREATED RATS

Autor Isokawa, M. Google Scholar
Mello, LEAM Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Membrane properties and synaptic responses were analyzed in dentate granule cells in hippocampal slices prepared from pilocarpine-treated, chronically epileptic rats. Perforant path stimulation evoked a long-lasting excitatory postsynaptic potential (EPSP) with multiple spikes in a stimulus intensity-dependent fashion. the response was strongly facilitated by paired-pulse stimulation. Application of N-methyl-D-aspartate (NMDA) receptor antagonist, D-2-amino-5-phosphonovalerate (APV), not only blocked the paired pulse facilitation but also reduced the amplitude of the EPSP, indicating the involvement of the NMDA-receptor in synaptic responses of pilocarpine-treated dentate granule cells. Dendrites of these neurons showed loss of spines and beaded branches. These findings suggest that a degenerating dendrite could be a morphological substrate of neuronal hyperexcitability mediated by NMDA receptors, implicating possible in vivo glutamate toxicity as an underlying mechanism of chronic epilepsy.
Palavra-chave HIPPOCAMPAL SLICE
MEMBRANE PROPERTY
LONG-LASTING EPSP
SPINE LOSS
BEADED DENDRITE
Idioma Inglês
Data de publicação 1991-08-05
Publicado em Neuroscience Letters. Clare: Elsevier Sci Ireland Ltd, v. 129, n. 1, p. 69-73, 1991.
ISSN 0304-3940 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 69-73
Fonte http://dx.doi.org/10.1016/0304-3940(91)90722-6
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1991GA84700016
Endereço permanente http://repositorio.unifesp.br/handle/11600/25212

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