REINNERVATION of the TRANSPLANTED VAS-DEFERENS - DIFFERENTIAL RECOVERY of VARIOUS BIOCHEMICAL and PHARMACOLOGICAL PARAMETERS

REINNERVATION of the TRANSPLANTED VAS-DEFERENS - DIFFERENTIAL RECOVERY of VARIOUS BIOCHEMICAL and PHARMACOLOGICAL PARAMETERS

Autor Jurkiewicz, N. H. Google Scholar
Jurkiewicz, A. Google Scholar
Garcia, A. G. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Biochemical and pharmacologcial parameters were used to follow the innervation characteristics of the rat was deferens transplanted (T) to the caecum. After about 5 months, a regeneration of autonomic nerves was clearly shown: first, by a complete recovery of neuronal uptake, indicated by: a) potentiation by cocaine of epinephrine (EPI) dose-response curves (T = 1.47 +/- 0.25, controls (C) = 1.50 +/- 0.14 log units); b) reversion to normal levels of pD2 values for norepinephrine (NE) and EPI (T = 6.6. +/- 0.1; 7.0 +/- 0.1, and C = 6.4 +/- 0.1; 6.9 +/- 0.1, respectively); second, a partial restoration of nerve terminals, and corresponding pools of NE, which was seen through histofluorescence and was indicated by a percent increase of: a) NE content, 47% (T = 3.8 +/- 0.8, C = 8.5 +/- 0.7-mu-g/g); b) dopamine-beta-hydroxylase (DBH) activity, 37% (T = 136 +/- 80, C = 364 +/- 15 nmol(hr.g); c) release of NE by 57 mM-potassium, 23% (T = 33.0 +/- 12.0, C = 147 +/- 14 ng/g. 5 min). Yet, two peculiarities of denervated organs remained practically unchanged even after 5-month transplantation: NE supersensitivity, measured by the relative responsiveness (rho) ratio (T = 0.96 +/- 0.02, C = 0.69 +/- 0.03), and tyramine-induced contraction, that was recovered by only 14% (T = 10.0 +/- 2.4, C = 72.0 +/- 3.5 mm). This differential recovery of the forementioned parameters is discussed in the light of receptor mechanisms and functional changes following reinnervation.
Palavra-chave VASDEFERENS
TRANSPLANTATION
REINNERVATION
NEURONAL UPTAKE
DBH
NOREPINEPHRINE
COCAINE
ALPHA-ADRENOCEPTORS
Idioma Inglês
Data de publicação 1991-01-01
Publicado em Journal of Neural Transmission-general Section. Vienna: Springer-verlag Wien, v. 85, n. 2, p. 83-94, 1991.
ISSN 0300-9564 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 83-94
Fonte http://dx.doi.org/10.1007/BF01244701
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1991FW53700001
Endereço permanente http://repositorio.unifesp.br/handle/11600/25181

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