PURIFICATION of SOLUBLE HUMAN LYMPHOCYTE-T RECEPTORS for SHEEP ERYTHROCYTES

PURIFICATION of SOLUBLE HUMAN LYMPHOCYTE-T RECEPTORS for SHEEP ERYTHROCYTES

Autor Itano, E. N. Google Scholar
Ono, M. A. Google Scholar
Sumigawa, M. Google Scholar
Longo, I. M. Google Scholar
Moura, N. C. Google Scholar
Mendes, N. F. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Using a polyclonal heterologous anti-soluble E-receptor serum, we identified molecules of molecular weight circa 58,000 and 150,000.The soluble receptor molecule with molecular weight of approximately 58,000 (R(s)1) was initially purified from supernatant of heated lymphocytes through chromatography on Sephadex G-200 and/or DEAE-cellulose. the soluble receptor molecule with molecular weight of approximately 150,000 (R(s)2) is detected at high levels in the serum of patients with cancer and uremia. R(s)1 and R(s)2 present in serum from cancer patients were purified by chromatography on Sephadex G-200 and by affinity chromatography using anti-R(s)1 IgG.I-131-labelled supernatant of heated lymphocytes binds to sheep erythrocytes and the elution and analysis of the molecules adsorbed showed bands of molecular weights approximately 58,000 and 150,000, confirming the receptor activity of these molecules.
Palavra-chave E-RECEPTORS
CD2
CHROMATOGRAPHY
Idioma Inglês
Data de publicação 1991-01-01
Publicado em Journal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 5, n. 3, p. 162-167, 1991.
ISSN 0887-8013 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 162-167
Fonte http://dx.doi.org/10.1002/jcla.1860050303
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1991FJ82600002
Endereço permanente http://repositorio.unifesp.br/handle/11600/25177

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