Experimental model of the morphological and morphometric aspects of tissue repair in skin wounds submitted to beta radiation emitted by Strontium -90 in rats

Experimental model of the morphological and morphometric aspects of tissue repair in skin wounds submitted to beta radiation emitted by Strontium -90 in rats

Alternative title Modelo experimental dos aspectos morfológicos morfométricos da reparação tecidual de feridas cutâneas submetidas à radiação beta do Estrontio-90
Author Pereira Filho, Gerson Vilhena Autor UNIFESP Google Scholar
Camelo-nunes, José Mario Google Scholar
Yui, Fabrício Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
ABC Medical School
Abstract The purperose of this work is tell the use of the experimental model to investigation of the effects of Beta radiation of estroncium-90 for repairing the tissue of wounds made in rats. 48 animals of the lineage EPM-1 Wistar were used, distributed in two groups that received radiation in alternate days (group A) and daily (group B). Each group was divided in four groups of six rats to be analysed in the 3rd, 7th, 14th and 21st day after operation. Two incisions were made in the back of the animal and sutured immediately afterwards; the upper part was irradiated and the lower part used as control. On the dates established the irradiated and control wounds were macroscopically examined and withdrawn for preparation of the histological comparative study in the optical microscope. Following, an morphometrical analysis was performed to count leucocyts, fiberblast and colagen fibers which were submitted to statistical study.

O objetivo deste trabalho é relatar o uso do modelo experimental para investigação dos efeitos da radiação Beta do Estrôncio-90 no processo de reparação tecidual de feridas provocadas em ratos. Utilizamos 48 animais da linhagem EPM-1 Wistar, distribuídos em dois grupos que receberam a radiação em dias alternados (Grupo A) e diariamente (Grupo B). Cada grupo foi subdividido em quatro, cada um com 6 ratos, para estudo no 3º, 7º, 14º e 21º dias de pós-operatório. Realizou-se duas incisões no dorso de cada animal, suturou-se em seguida, sendo que a de situação cranial foi irradiada e a caudal serviu para controle. Nas datas determinadas, as feridas controle e irradiada foram observadas macroscopicamente e retiradas para preparo do estudo histológico comparativo ao microscópico óptico. Realizou-se em seguida, análise morfométrica para contagem de leucócitos, fibroblastos e fibras colágenas, submetidos a estudo estatístico.
Keywords Models, Animal
Wound healing
Beta radiation
Rats
Histological study
Modelos animais
Reparação tecidual
Radiação beta
Ratos
Estudo histológico
Language English
Date 2004-12-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 19, p. 51-58, 2004.
ISSN 0102-8650 (Sherpa/Romeo)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 51-58
Origin http://dx.doi.org/10.1590/S0102-86502004000700010
Access rights Open access Open Access
Type Article
SciELO ID S0102-86502004000700010 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/2331

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