Guinea pigs as experimental model to evaluate the resistance of the tissue expander capsule

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dc.contributor.author Nahas, Fabio Xerfan [UNIFESP]
dc.contributor.author Vasconez, Luis O.
dc.contributor.author Ferreira, Lydia Masako [UNIFESP]
dc.date.accessioned 2015-06-14T13:31:22Z
dc.date.available 2015-06-14T13:31:22Z
dc.date.issued 2004-12-01
dc.identifier http://dx.doi.org/10.1590/S0102-86502004000700016
dc.identifier.citation Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 19, p. 96-103, 2004.
dc.identifier.issn 0102-8650
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/2327
dc.description.abstract The purpose of this study is to report the use of guinea pigs as experimental model to study the resitance of the tissue expander capsule. Two groups were studied. In both groups a round 20 cc tissue expander, attached to a multiperforated catheter was inserted. The pocket housing was standardized. The skin over the expanded area was tattooed demarcating the area and arc, which were measured before and after each expansion. Every 4 days, a volume corresponding to 10% of the expander's total capacity was injected. Animals of the control group received 3 cc of saline through the catheter at the same time of expansion. Animals of the experimental group received 3 cc of the HBGF-1 diluted with saline. The intraluminal pressure of the expander was measured before and after the injection. After its total filling, the animals were sacrificed and 5 cc more were injected into each expander. The pressure was measured after each 1 cc injected. The capsule was examined histologically by immunofluorescence, trichrome and hematoxylin-eosin stains with the purpose of quantifying collagen and fibroblasts. The experimental model to study the resistance of the tissue expander's capsule showed to be feasible in guinea pigs. en
dc.description.abstract O objetivo deste estudo é relatar o uso de cobaias como modelo experimental para estudar a resistência da cápsula de expansores. Dois grupos foram estudados. Em ambos os grupos um expansor Redondo de 20 ml com um cateter multiperforado fixadoforam inseridos nos animais. A loja foi padronizada. A pele a ser expandida foi tatuada demarcando uma area e um arco que foram medidos a cada expansão. A cada 4 dias. Um volume correspondendo a 10 % da capacidade total do expansor foi injetado. Os animais do grupo controle receberam 3 ml de soro fisiológico pelo catéter de irrigação no mesmo momento da expansão. Os animais do grupo experimental receberam 3 ml de HBGF-1 diluido com soro fisiológico. A pressão intraluminal do expansor foi medida antes e após a injeção. Após o prenchimento total do expansor, os animais foram sacrificados e mais 5 ml foram injetados dentro de cada expansor. A pressão foi medida após cada 1 ml injetado. A cápsula foi examinada histologicamente por immunofluorescência, tricromo e hematoxilina-eosina com a finalidade de quantificar colágeno e fibroblastos. O modelo experimental para estudar a resistência da cápsula do expansor de pele mostrou-se factível em cobaias. pt
dc.format.extent 96-103
dc.language.iso eng
dc.publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Guinea pigs en
dc.subject Capsular contracture en
dc.subject Tissue expander en
dc.subject HBGF-1 en
dc.subject Cobaia pt
dc.subject Contratura capsular pt
dc.subject Expansor de pele pt
dc.subject HBGF-1 pt
dc.title Guinea pigs as experimental model to evaluate the resistance of the tissue expander capsule en
dc.title.alternative Cobaias como modelo experimental para avaliar a resistência da cápsula do tecido expandido pt
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Alabama University Plastic Surgery Division
dc.description.affiliation Federal University of São Paulo EPM Surgery Department
dc.description.affiliation Alabama University Plastic Surgery Division
dc.description.affiliationUnifesp UNIFESP, EPM, Surgery Department
dc.identifier.file S0102-86502004000700016.pdf
dc.identifier.scielo S0102-86502004000700016
dc.identifier.doi 10.1590/S0102-86502004000700016
dc.description.source SciELO



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