Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor

Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor

Autor Lima, Eleonidas Moura Google Scholar
Rissino, Jorge Dores Google Scholar
Harada, Maria Lucia Google Scholar
Assumpção, Paulo Pimentel Autor UNIFESP Google Scholar
Demachki, Samia Google Scholar
Guimarães, Adriana Costa Google Scholar
Casartelli, Carla Autor UNIFESP Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
Burbano, Rommel Rodríguez Autor UNIFESP Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal do Pará Centro de Ciências Biológicas Departamento de Biologia
Universidade Federal do Pará Centro de Ciências Biológicas Departamento de Genética
Universidade Federal do Pará Hospital Universitário João de Barros Barreto Departamento de Patologia e Serviço de Cirurgia
Universidade Federal de São Paulo (UNIFESP)
Resumo Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0) originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different origins have been treated with several conventional and investigational anticancer drugs. The ACP01 cell line is triploid, grows as a single, non-organized layer, similar to fibroblasts, with focus formation, heterogeneous division, and a cell cycle of approximately 40 h. Chromosome 8 trisomy, present in 60% of the cells, was the most frequent cytogenetic alteration. These data lead us to propose a multifactorial triggering of gastric cancer which evolves over multiple stages involving progressive genetic changes and clonal expansion.
Palavra-chave Cell line ACP01
Human gastric adenocarcinoma
In vitro immortalization
Chromosome 8 trisomy
Clonal expansion
Idioma Inglês
Data de publicação 2004-12-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 12, p. 1831-1838, 2004.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 1831-1838
Fonte http://dx.doi.org/10.1590/S0100-879X2004001200008
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000225509100008
SciELO S0100-879X2004001200008 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/2318

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