Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells

Colocalization of coilin and nucleolar proteins in Cajal body-like structures of micronucleated PtK2 cells

Autor Silva, Neusa Pereira da Autor UNIFESP Google Scholar
Christofolini, Denise Maria Autor UNIFESP Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Andrade, Luiz Eduardo Coelho Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK2 cells undergoing micronucleation. Nocodazol-induced micronucleated cells were examined by double indirect immunofluorescence with antibodies against coilin, fibrillarin, NOR-90/hUBF, RNA polymerase I, PM/Scl, and To/Th. Cells were imaged on a BioRad 1024-UV confocal system attached to a Zeiss Axiovert 100 microscope. Since PtK2 cells possess only one nucleolus organizer region, micronucleated cells presented only one or two micronuclei containing nucleolus. By confocal microscopy we showed that in most micronuclei lacking a typical nucleolus a variable number of round structures were stained by antibodies against fibrillarin, NOR-90/hUBF protein, and coilin. These bodies were regarded as CB-like structures and were not stained by anti-PM/Scl and anti-To/Th antibodies. Anti-RNA polymerase I antibodies also reacted with CB-like structures in some micronuclei lacking nucleolus. The demonstration that a set of proteins involved in RNA/RNP biogenesis, namely coilin, fibrillarin, NOR-90/hUBF, and RNA polymerase I gather in CB-like structures present in nucleoli-devoid micronuclei may contribute to shed some light into the understanding of CB function.
Palavra-chave Cajal body
Coiled body
Micronuclei
Cell nucleolus
Nucleolus organizer region
RNA polymerase I
Nucleolar proteins
Idioma Inglês
Data de publicação 2004-07-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 7, p. 997-1003, 2004.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 997-1003
Fonte http://dx.doi.org/10.1590/S0100-879X2004000700008
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000222450200008
SciELO S0100-879X2004000700008 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/2156

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