An experimental model of mycobacterial infection under corneal flaps

An experimental model of mycobacterial infection under corneal flaps

Autor Adan, Consuelo Bueno Diniz Autor UNIFESP Google Scholar
Sato, Elcio Hideo Autor UNIFESP Google Scholar
Sousa, Luciene Barbosa de Autor UNIFESP Google Scholar
Oliveira, Rosangela Siqueira de Autor UNIFESP Google Scholar
Leao, Sylvia Cardoso Autor UNIFESP Google Scholar
Freitas, Denise de Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo In order to develop a new experimental animal model of infection with Mycobacterium chelonae in keratomileusis, we conducted a double-blind prospective study on 24 adult male New Zealand rabbits. One eye of each rabbit was submitted to automatic lamellar keratotomy with the automatic corneal shaper under general anesthesia. Eyes were immunosuppressed by a single local injection of methyl prednisolone. Twelve animals were inoculated into the keratomileusis interface with 1 µl of 10(6) heat-inactivated bacteria (heat-inactivated inoculum controls) and 12 with 1 µl of 10(6) live bacteria. Trimethoprim drops (0.1%, w/v) were used as prophylaxis for the surgical procedure every 4 h (50 µl, qid). Animals were examined by 2 observers under a slit lamp on the 1st, 3rd, 5th, 7th, 11th, 16th, and 23rd postoperative days. Slit lamp photographs were taken to document clinical signs. Animals were sacrificed when corneal disease was detected and corneal samples were taken for microbiological analysis. Eleven of 12 experimental rabbits developed corneal disease, and M. chelonae could be isolated from nine rabbits. Eleven of the 12 controls receiving a heat-inactivated inoculum did not develop corneal disease. M. chelonae was not isolated from any of the control rabbits receiving a heat-inactivated inoculum, or from the healthy cornea of control rabbits. Corneal infection by M. chelonae was successfully induced in rabbits submitted to keratomileusis. To our knowledge, this is the first animal model of M. chelonae infection following corneal flaps for refractive surgery to be described in the literature and can be used for the analysis of therapeutic responses.
Assunto Infectious eye diseases
Keratitis
Animal model
Mycobacterium chelonae
Lamellar keratotomy
Idioma Inglês
Data 2004-07-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 7, p. 1015-1021, 2004.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Editor Associação Brasileira de Divulgação Científica
Extensão 1015-1021
Fonte http://dx.doi.org/10.1590/S0100-879X2004000700010
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000222450200010
SciELO S0100-879X2004000700010 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/handle/11600/2152

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